Photosynthesis Lab Report
Energy Lab Assignment: Photosynthesis
Abstract
The purpose of this experiment was to determine whether film on white light had an impact on the transmittance percentage and to establish whether the film's color was relevant to the outcomes. In this experiment, the photosynthetic absorbance of spinach thylakoids was measured using a spectrophotometer. The hypothesis was that white light would have the highest transmittance percentage compared to green film on white light and red film on white light.
Introduction
Photosynthesis is the process through which light energy is turned to chemical bond energy. Autotrophic organisms need photosynthesis to survive. The cells that are able to carry out photosynthesis engage in a variety of critical
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The objective of this experiment is to determine how light affects photosynthesis. The purpose of this experiment is to determine whether or not colored films have any impact on the rate of transmittance when used with white light.
In this experiment, there were three treatments. One was white light that was 60 watt. The other two had films on the white light, green film and red film. We used the thylakoids of spinach and they were blended with 0.5 M of sucrose. In three different test tubes, we poured 150L of thylakoids mixture and then we poured them into cuvettes. We also used a spectrophotometer to measure the transmittance reading. As soon as we placed the cuvette into the spectrophotometer, we took the reading and placed it in front of the light source for 30 seconds.
I hypothesize that the percentage of transmittance will be higher for white light than the different colored films and that the color of the films have an effect on the percentage too. The film on the light hinders the transmittance and since green light is already reflected by plants, I felt that the green film would have a lower transmittance than the red
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My findings showed that the green film on white light constantly has a slightly higher transmittance percent than the red film. Half of my hypothesis was supported while the other half was rejected. The white light was most effective at driving photosynthesis. The transmittance readings for white light was slightly higher than green film on white light and much higher than red film on white light. Since green film is reflected by chlorophyll during photosynthesis, these transmittance readings surprised me that the green film had a higher slope than the red film. It’s possible that the red film on the light is the reason for the transmittance reading below which could have made the white light
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