Photosynthesis Lab Report

Task 1 M1 Describe the scientific principles behind each of the three procedure above. Vacuum filtration is a procedure when a sold needs separating from a solvent to react the mixture. Then the mixture of a solid is measured through the filtration paper in a Buhner funnel. The liquid is drained through the funnel into the flask.

Then, 100mL 6M HCl was added to the same beaker also by using a graduated cylinder. The solution was stirred with a stirring rod. To make the 2M NaOH solution, 50mL deionized water was added to a 400mL beaker labelled “2M NaOH”. Then, 100mL 3M NaOH was added to the same beaker.

The resin that had stuck to the sides of the burette was washed down by pipetting extra pH 3 citrate buffer along the sides. The column was tapped to ensure that the settled resin formed a level surface. After all of the resin settled, the buffer was drained into a waster beaker until the level of the buffer reached the top surface of the resin. For the remainder of the experiment, the top surface of the resin was not allowed to dry

Experiment 1: Materials: • Alka-Seltzer tablets • Empty and clean water or soda bottles (12 oz to 24 oz) • Balloons • Water • Clock • Stove top Procedure: 1. Pour a sufficient amount of water (about 16 oz) into a small pot and place on the stove at high heat. 2. Watch the clock and after 30 seconds take the water off the heat.

The 0.1% is the concentration amount. Just like temperature and pH, substrate concentration can speed the reaction only up to a certain limit. When we mixed pH 3 enzyme tube with substrate tube, we used 0.3 mL of hydrogen peroxide, but if we were to increase the amount, then the experiment would have been faster. Our

We then weighed a filter paper which we will use later in the experiment. We then collected the crystallized acetylsalicylic acid by vacuum filtration in a Buchner funnel and washed the product with a little ice-cold water. We then pre-weighed a clean, empty watch glass and labelled it with our initials and the date, we did this do we could easily identify that it was ours when we go to weigh it with the crystals on. We

However, the R squared value is 0.929, meaning that it is close to fitting the line of best fit. As the amount of alpha galactosidase increased, the glucose concentration also increased. This resulted in the positive trend of the graph and a steeper slope. When 4 mL of alpha galactosidase was added to the bean solution, the result evened out, and didn’t change as drastically

Doriana Spurrell What different frequencies and types of light would prompt the Spinach leaves to go through the process of photosynthesis effectively? Purpose: The purpose of this experiment was to see which of the four lights that range across the light spectrum would properly and most efficiently help the spinach leaf perform photosynthesis. Background Information: Photosynthesis is the process in which plants use light energy to transform into chemical energy.

Dislodge any bubbles by tapping on a hard surface and position the cuvette in the spectrometer so light passes through the clear side. 7. Connect the spectrometer to Labquest and select a new file. Calibrate the spectrometer by placing the blank inside and allowing the lamp to warm up. The optimal wavelength for the standard curve and data collection can be started at this stage.

Purpose: The purpose of this experiment is to see how long it takes for the 10 spinach leaf discs to undergo photosynthesis and thereby rise in the two solutions. Hypothesis: All of the leaf discs in the sodium bicarbonate solution should be floating before the discs in plain water because the bicarbonate is a carbon source that will allow photosynthesis to continue. Background: Light is absorbed by leaf pigments (chlorophyll) which makes electrons within a photosystem moved to a higher energy level.

Remove all salt from beaker into a plastic container “g”. Scrape off ALL extra salt to make sure you have everything(If salt isn’t completely dry then spread out and let the rest of the water evaporate on its own. Do not wash out beaker until you know that ALL of the salt is removed from it). Mass all of the separate substances (Beads, Iron, Salt, and Sand). Place plastic container “h” on the digital scale.

10. The solution was then placed under the fume hood for the chloroform to evaporate. 11. Methanol was filled in a test tube and placed into a water bath to heat up. 12

Purpose This experiment is to determine the concentration of the solute copper sulfate pentahydrate, and the unknown solution, by passing different wavelengths of light through each solution. Procedure Weigh out approximately 5g of copper sulfate pentahydrate. Record the mass and place the solute into a 50 mL volumetric flask. Fill half of the flask with distilled water, add the stopper for the flask, and lightly shake the flask, until the copper sulfate pentahydrate fully dissolved.