Plant Tissue Culture Essay

NANYANG RESEARCH PROGRAMME

Progress Report - Searching for a Thermal- or Photon- Remedy for Plant Regeneration (NIE02)
Supervisor: Associate Professor Chen Zhong
Name of Student: Le Van Tuan Long
School: Temasek Junior College

Introduction:
This research makes use of plant tissue culture. Plant tissue culture is commonly used to propagate plants in sterile conditions, often to produce clones of plants. Different methods and techniques in plant tissue culture offer different advantages in plants research and development. A few of the advantages are:
• Production of exact copies of plants that produce particularly desirable traits
• Quickly produce mature plants as the medium are strictly controlled
• Produce multiple plants without

Yellow is unhealthy, green is more healthy
 Marchantia are best grown in Square Tissue Culture Vessel (allows plants to grow in the upright position)
 Marchantia doesn’t have seeds. They grow from cut-out branches
 Fertilisation of male and female will produce spores which is the next generation of the marchantia)

o First Skill: Fertilise female Marchantia using sperms of male Marchantia:
 Transfer female Marchantia branches using forceps into another circular plate/square vessel with the B5 medium (Some plants may be connected to each other, must separate)
 Sometimes the forceps may be dirty, dip in 70% ethanol solution (clean forceps near the handle also as it may be contaminated there)
 Cap the bottle and cover the plates when not in use to prevent contamination
 If the branches are too tall, don’t choose those.
 5-7 branches per plate/vessel will do
 Obtain sperms from male Marchantia
 Using a small tip (yellow), put a droplet of dd water onto the umbrella of the make Marchantia sec organ (*Replace the tip after the water run out, don’t dip into the bottle again as it will contaminate the dd water