Dip a sample's swab into that individual sample and do five strokes over the agar in the half of the petri dish. On the other half, leave it alone to serve as a control to determine how much bacteria ends up growing on its own. On the outside of the petri dish use a sharpie to draw a line dividing the two sides and leave the controlled side blank. 5. Repeat step four for each sample with a new sterile swab each time.
Superflab bolus material with the dimensions 10 cm x 10 cm x 1.0 cm is placed flush on top of the solid water to obtain the Dmax. Subsequent measurements made with Play-Doh which placed on top of a clear thin plastic bag, as well as the rice in a sandwich bag having the same dimensions as Superflab. The same set of trials performed with the neon green Play-Doh and the orange Play-Doh. A Varian Clinac 6EX Linear Accelerator produced 6 MV photons and irradiated a field size of 10 cm x 10 cm for each dose. Two hundred monitor units (MU) administered during each trial on a clinically calibrated linear accelerator.
Using a disposable plastic pipet, worms were transferred with a bit of spring water to the viewing chamber and given a few minutes to settle to their new surroundings. The viewing chamber was then placed under the dissecting microscope at the lowest power, which helped with focusing on the middle body region of the worm to measure pulsation rates. Using a stopwatch, the basal rate of the worm was obtained by counting the number of pulses that moved through a segment in a thirty second interval, this amount was multiplied by two to result in units of beats per minute. Three basal rates were recorded for each of the three individuals warms to calculate their mean rate. Worms A, B and C were then placed into separate containers containing the caffeine treatment solution.
Stock culture: About 5,000 adult flies were maintained in steel framed cages (76 66 76 cm) covered with wired net. The flies were supplied with protein based artificial diets viz., (i) baking yeast: sugar: water at 1:3:4 ratio, and (ii) casein: yeast extract: sugar at 1:1:2 ratio. Water was supplied in a conical flask socked with cotton ball. Temperature (°C) and relative humidity (RH) of the rearing room was maintained at 27 ± 2°C and 75 ± 5%, respectively by using air conditioner (Model No. Movincool Classic Plus 26, USA).
Then smear the dipped cotton tipped applicator onto the surface that you want to collect a culture from c. Gently roll the cotton tipped applicator into the gel appropriately labeled quarter of the petri dish. d. Throw the used cotton tipped applicator away e. Repeat a – d with three other surfaces. 7. Take a piece of parafilm and secure the side of the petri dish 8. Store the petri dish in the incubator Data: Day 1 Dish was spotted with slight growth of bacteria.
A : Not at all, the Hamilton Beach Juice Extractor is excellent at juicing soya beans and almond. However, it must be soaked in water for 24 - 48hours. One of cup of rice, soybean, and almond is soaked in 4 cups of water and placed in a refrigerator. Additionally, soybean can be boiled a bit to release the flavors. Q: Does it have a manufacturer warranty?
Table 2 illustrate major carbohydrates in buckwheat honey. As can be seen, six kinds of carbohydrate were distinguished in all the honey samples, including fructose, glucose, kestose, isomaltose, isomaltotriose, panose. Fructose was likely to show the highest level ranged from 40.4% to 48.7%. The second-high content was glucose ranged 21.6% to 28.7%. Kestose, isomaltose, isomaltotriose, panose occurred in all buckwheat honey samples.
Rats were sacrificed at different periods by cervical dislocation, dissected and the adrenals free of adhering fat were used for analysis. The tissues were stored at -200C after weighing whenever necessary. Out of the two adrenals in each animal, the left adrenal was analysed for peroxidase and the right for AA. Until & otherwise mentioned, five replicates at a time were used for each
To measure the accurate time when the membrane will break Size and type of the beetroot (size of beetroot) Used the same beetroot for the entire experiment. By using the cork borer and the ruler we ensured that each beetroot core has the same diameter and length. (check the diameter of the cork borer) Fair experiment (accuracy) Volume of ethanol (10ml) 10 ml of ethanol solution was used for each experiment (except tube 1 for 100% ethanol concentration 20ml was used) Pipette is used for accurate
Each group comprised of 6 replicates with 9 chicks per replicate. The total experimental duration was 5 weeks. The diet was prepared using maize and soybean as major ingredients for pre-starter (0-1 wk), starter (2-3 wk) and finisher (4-5wk) periods (Table 1) and offered ad libitum with access to clean drinking water. Growth Performance Body weight changes were recorded every week to ascertain the weekly and overall body weight gain. The experimental diets were given ad lib and the residue was weighed at weekly interval in order to arrive at feed intake.
50 mL of tap water was obtained in a small beaker and then was slowly moved into the lid of the bottle using a funnel. The sugar used in this experiment was created by mixing ten milliliters of starch,glucose, and regular countertop sugar. This was transferred into the joint water bottle tunnel using a funnel. Both ends of the tunnel were sealed shut and each measurements were taken every three minutes and final measurements were taken after 21 minutes. The results were
Chipotle-Thyme Black Beans There are actually 5 different varieties of black beans. But when you purchase black beans, they are often just labeled as “black beans.” Serves 8 Ingredients 2 cups dried black beans 16 cups water 1 tablespoon vegetable oil 1 teaspoon chipotle powder 2 teaspoons fresh thyme, minced 1 teaspoon salt 1.Add the beans and 8 cups water to the pressure cooker. Lock the lid into place; bring to high pressure for 1 minute. Remove from the heat and quick- release the pressure. 2.Drain the water, rinse the beans, and add to the pressure cooker again with the remaining 8 cups water.
In this experiment, we devided each of the different beans. We used mug, black, and coffee beans. By placeing a plate for each bean, We where able dividing each the mug, black, and coffee beans into each plate. After dividing each bean we labled the name and group so we can better understand the difrence between the beans. The meathod of division is very simple and organized, alows to diffrentiate the difrence between each bean.
The Kettle chips are natural and have about 120 calories. Serving size 1oz about 20 chips, 3g of total fat, 150mg of sodium and has lots of potassium about 410mg. Something similar to Kettle Chips is Beanitos. Beanitos has 120 calories. Serving size 1oz, serving per container 3, total fat 4g.
All of the plates were looked at for the amount of colonies grown, if growth was present, and if the colonies gained the ability to glow green. Transformation was successful in the plates where the bacteria consumed the pGLO plasmid. In the first plate that the bacterium was plated on it included the LB broth and of ampicillin antibiotic (amp), 2 colonies were present. The second plate of bacteria was grown with the presence of LB broth, ampicillin, arabinose sugar (ara), and 22 colonies were observed. But a green fluorescent glow of the colonies was only present in plate 2.