Pomegranate Peel Lab Report

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Standardization of the processing conditions for the extraction of polyphenols from pomegranate peel (Punica granatum) Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore-570020 (India) Venkataramanamma, D., Aruna, P., Ravendra P. Singh* * Corresponding author, Ph: +91-821-2514876, FAX : +91-821-2517233, e mail singhrp317@gmail.com ABSTRACT Pomegranate peel is known to possess higher antioxidant activity than the edible portion. An attempt has been made to obtain peel extract - at room temperature using combination of water and ethanol and the factors, which may affect the extraction efficiency have been optimized. The storage stability of the extract has been studied for >100 days at room temperature (30-32), 4, 0 and -80˚C. The extract quality was monitored by measuring the radical scavenging activity (RSA) by diphenylpicrylhydrazyl (DPPH)…show more content…
The peel powder was soaked overnight (or for desired period) at room temperature (30-32°C) for extraction with intermittent shaking. After extraction, it was centrifuged at 2000 rpm for 5 min and filtered through Whatman No. 2 filter paper. The effective volume obtained after centrifugation was noted. 2.3 Determination of radical scavenging activity (RSA) Determination of RSA was carried out according to Murthy, Jayaprakasha, & Singh (2002) using DPPH as stable free radical and butylatedhydroxyanisole (BHA) as standard. Different aliquots of extract and BHA were taken in 100 μl fixed volume. 0.1 mM methanolic solution of DPPH (5 ml) was added and shaken vigorously. The tubes were kept at room temperature in dark for 20 min. MeOH was used for baseline correction. The changes in the absorbance were measured at 517 nm (Genesys-5, Milton Roy, USA). RSA was expressed as percentage inhibition using the formula. % RSA = control OD−sample OD/control OD X

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