Pomegranates Lab Report

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Two varieties of pomegranates named Rabab and Hasibi which are harvesting in Yazd (Iran), have been used. Fruits were peeled and dried up under sunlight. They were washed with sterile distilled water. The dried rinds were grinded into a fine powder by Mixing grinder.
The aqueous extract was prepared by dissolving 1g of dry extract with 20 ml of sterilized distilled water, so the final concentration of extract would be 0.05 g/ml, from this solution other concentration were prepared (0.1-0.2) g/ml. the solutions were shaken for 30 min. The extract was centrifuged (30,000 rpm; 15 min) and the supernatant was Separated. To hydroalcoholic extract, 80 g of the powder was extracted with aqueous methanol (75%). The other two concentrations were prepared from soaking sixfold aqueous methanol (75%) with different amounts of powder. At the end of extraction each extract was passed through the Whatman filter paper No. 1 (Whatman, UK). The methanol was removed from residue with rotatory evaporator (نام دستگاه، سال و .. ).
Agar well diffusion method
This method was used to determine the antibacterial activity of the extracts. For preparation of the inoculum including overnight culture of strains (Staphylococcus aureus(ATCC 29737), methicillin resistance Staphylococcus aureus(MRSA)(ATCC 33591) and
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primarily each isolate was cultured in broth medium and incubated on 37 C. overnight. Suspensions of any isolates were prepared that turbidity equal to 0.5 McFarland standards(OD=0.1). Each bacterium had been swabbed with those suspensions on Muller Hinton agar medium. Four wells were created (6mm in diameter and the distance of 25cm from each other) with sterile cork borer. Each well was poured with 100 μl clear supernatant liquid of any concentration. Plates were placed in room temperature to absorb extracts. They incubated for 24 hours at 37C. their diameter of inhibition zone was measured in

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