Poppy Seed Essay

2000 Words8 Pages
Ankita Sharma1, Parul Sharma2 and Nimali Singh3
1. Research Scholar, Department of Home Science, University of Rajasthan; 2. Assistant Professor, Department of Food Science and Nutrition, Banasthali University; 3. Associate Professor, Department of Home Science, University of Rajasthan, Jaipur ABSTRACT
INTRODUCTION: Poppy seed is an oilseed obtained from the opium plant (Papaver somniferum). It is one of the oldest known painkillers consisting of many alkaloids (morphine, codeine etc.) and is rich in protein and calcium. The morphine content of poppy seed intended for use in food recently have raised major concern due to intoxication
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It is an erect annual herb which has been cultivated from prehistoric times (Bernath et al., 2009). The production of poppy principally occurs in northern India in the region of Madhya Pradesh and Rajasthan (Schiff, 2002; Srinivas and Rao, 1981). It is one of the oldest known painkillers and is the source of many alkaloids which includes narcotics and analgesic morphine and the codeine, thebaine, nonscapine and many more. (Shukla et. al., 1995; shukla et al., 2006; yadav et al., 2006). The morphine content of poppy seed which is widely used in dishes and pastries has recently raised major concerns (Sproll et al, 2006) and cases of intoxication after consumption of strongly contaminated poppy seed even led to a discussion about maximum limits. The federal institute for risk assessment (BFR) derived a provisional guidance value of 4mg morphine/kg poppy seed based on a maximum daily intake of 0.38mg morphine/person. The maximum value of 4mg morphine/kg poppy seed might have led to a nearly complete market shake out because a great deal of all products had concentration above the limit. To recalibrate this systematically the research was done to determine the influence of food processing and the significant reduction of morphine (during steps like grinding and boiling) was observed (Sproll et al,…show more content…
Then the mixture was stirred with vortex mixture for 1 min and centrifuged (18000xg, 10 min). The supernatant (20µl) was injected into the HPLC column.
Estimation of Alkaloids: - The opium extract sample were analysed by paired ion reverse phase Chromatography following Lurie waters (Milford, U.S.A). High pressure liquid chromatography (model ALC/GPC-204) consisting of M 6000 A solvent delivery system, U6 K injector, µ Bondapack C18 column (4mnid x 30 cm), M 440 UV detector at 254 nm and 10 mv recorder (Houston Instruments, Houston, Texas) was used for analysis.
The opium samples for chromatography were prepared by titrating 75 mg of dry opium in 10ml of dimethyl sulphoxide (DMSO) for about 10 minutes. This was followed by ordinary filtration and then running the samples through waters samples classification kit, the injection volume was

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