The three things that can cause the enzyme to denature is a large change in pH level, High Temperature, and substrate concentration. According to our knowledge, we know that a large change in pH will cause instability in the protein structure thus resulting in denaturation of the enzyme. From the data, we can see that pH 3 (total:6.3) and 10 (total:6.2) were the slowest because pH 3 is probably the highest acid and pH 10 is the highest base. The highest acid or base pH represents a large change which would cause the enzyme to denature. The fastest pH was 6 (total:34.5), and it seems that there wasn’t a large change which resulted in a stable structure.
Task 1 M1 Describe the scientific principles behind each of the three procedure above. Vacuum filtration is a procedure when a sold needs separating from a solvent to react the mixture. Then the mixture of a solid is measured through the filtration paper in a Buhner funnel. The liquid is drained through the funnel into the flask.
Some research has indicated that a lack of catalase can lead to the development of type 2 diabetes. It seems that some other molecules within living organisms are able to sufficiently break down hydrogen peroxide—enough to sustain life. The toxic nature of hydrogen peroxide also makes it a powerful disinfectant. And in conclusion from the information ive found ,catalase functions best at around 37 degrees Celsius as the temperature gets colder or hotter than that, the ability to work will denature and the enzyme will be
(see table #2) The mixture with the bean water caused the solution to not be as concentrated, limiting the amount of oligosaccharides that the alpha galactosidase can break down, therefore resulting in a small amount of glucose concentration. The highest stand standard deviation is at 4 mL of alpha galactosidase, which is 185.742. The lowest standard deviation is at 0 mL and 1 mL of alpha galactosidase, which is 0. Since error bars are not all overlapping, it shows that there was a significant difference (see figure #3).
Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes.
The experimental hypothesis for this experiment was prove as the results from the experiment provide evidence to suggest so. The osmosis process was shown when the potato cylinders were submerged in the salt solution and over time this only became more prominent. Looking at graph A, the potato cylinders submerged in the water solution gained mass and became harder when pressure was applied. This meaning no osmosis happened as expect as the mass gain implies there was just passive transport. The water molecules in the water solution were small enough to pass through the semi permeable membrane in order to equalise so there was a balance between the potato and the water solution.
A Keto diet is a low carb diet. Because there are not enough carbohydrates to energize the body, the body will begin to break down fat as an energy source, which in turn causes weight loss. When you starve yourself, you are at risk for ketoacidosis, which causes high pH levels in the blood. We modeled this diet to see the effect it had on enzymes. To represent the blood of a person with ketoacidosis, we used a liquid that had a high pH level.
Part 2 . Reactions with HCl Both magnesium and calcium were repeated placing in 3 ml of 6M HCl. Both solution released colourless gas bubble after placing the metals in the test tubes. Both solutions were tested with burning wooden splinter placing near to the mouth of the test tubes, both of the test tubes produced a ‘pop’ sound.
After we set the spectrophotometer to zero, we mixed the second enzyme with the second substrate and promptly poured it into a clean cuvette to then be put in the spectrophotometer and record the absorbance at zero seconds, and again every thirty second for three
The solution that have a molarity of 0, cause the object to be isotonic. If the temperature of the glucose solutions that had different molarities increased, the reaction of the potatoes weight would have happened faster. And vice versa, if the temperature of the glucose solutions that had different molarities decreased, then the reaction of the potatoes weight would have happened slower. If someone used animal cells instead of plant cells in this experiment, nothing would change because the only difference between plant cells and animal cells is the cytoplasm which has nothing to do with the experiment. A solution that is five percent glucose would be isotonic compared to red blood cells.
After the loop cooled down, the loop was usedd to obtain a sample of the unknown mixture. This done by inserting the loop in the test tube of the unknown mixture. The loop was now ready to begin streaking; the plate was divided into four quadrants. Streaking was initiated into the first quadrant using a side-to-side swipe method. After streaking into the first quadrant, place the loop through the flame for 3-5second in order to reduce the amount of bacteria
In conclusion, from the collected data, the lack of the change in characteristics from Biuret’s solution suggests that there is a minimal amount of protein in both of the McDonald’s happy meals, if
Next one microliter of DNA was mixed with one microliter of loading dye using a pipettor and loaded into the well. The same mixing process was completed for the PCR product, using one microliter of PRC product and one microliter of loading dye. For the purposes of this experiment, the DNA product was loaded into well six and the PCR product was loaded into well seven. Initially DNA was loaded into well five, however gel was pierced so samples were moved one well to the right. The gel was run at 100 V for one hour.
Materials I used. Were a petri dish, scapula, liquids, burner, C-clamp, tongs, gas to start the flame, eight different types of powders, and the most importa martial was our goggles for safety. The petri dish was used to put the powder in for testing. The scapula was used for scooping up the powders.
While in the room I gave the blood draw specimen box to RN Anglea Wynn. She opened the box and removed the contents. I removed the right cuff from Mr. Defalco’s arm so blood could be taken. She used the betadine in the box to clean the area where the blood would be taken. At 4:29 a.m. blood was drawn, RN Wynn placed the blood in the tubes provided in the box.