Research Proposal
Title: Development of a micro-particulate system for probiotic bacteria to increase their survival and viability through pasteurization, UHT and storage conditions of dairy products. Justification:
Probiotics are living microorganisms that are believed to provide many health benefits to humans. According to international dairy federation (IDF), a probiotic food product should contain at least 107 CFU/g of beneficial bacteria at the time of consumption. In the recent years products fortified with probiotic bacteria has flooded the consumer market, and most of these products are dairy products. Yoghurt, Cheese, Ice cream, infant formula, milk powder, and viscous dairy beverages are some of the most popular carriers of probiotic bacteria.
All dairy products are pasteurized to eliminate vegetative cells of pathogenic and spoilage bacteria. The dairy processors make use of different time-temperature combinations to provide this thermal treatment. For example LTLT [low temperature low time, 63°C for 30 min], HTST [high temperature short time 72°C for 15 s], 89°C for 1 s, 90°C for 0.5 s, 94°C for 0.1 s, 100°C for 0.01 s are some of the most common
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Develop microencapsulated system for probiotic bacteria utilizing appropriate microencapsulation techniques and using combination of encapsulating materials having good barrier properties against heat (heat resistant, low thermal conductivity). (2).Evaluate physical, chemical and microbiological properties of microcapsules. (3). Determine the effect of pasteurization and UHT treatments on survival rate of microencapsulated probiotic bacteria fortified in different types of dairy products (yoghurt, cheese, ice cream, milk powder, infant formula, dairy beverages). (4). Determine the effect of microcapsules on physico-chemical and sensory properties of finished dairy products.
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
A milk-based, litmus broth tube is incubated and observed after 48 hours. Observations include lactose fermentation without gas as well as with gas, the reduction of litmus, casein protein coagulation and casein and protein hydrolysis. These characteristics were all determined based on the color of the solution and the production of a curd, the curds density and the production of a gas. To determine the density of the curd, the tube was slightly turned to see rather or not it was mobile or concentrated towards the bottom. 2.3 Carbohydrate Fermentation of Lactose, Sucrose and
Food is the sustaining life force that drives the human race forwards from day to day. As daily consumers of food products, it is automatically expected that the producers of such important products aim to produce goods that will help the consumer and attribute to their health. Unfortunately, this is not always the case. At the turn of the twentieth century, food sanitation in factories was at an all time low. Adding to this issue were the harsh conditions in which the workers were forced to work in.
Although microscopic single-celled organisms inhabited earth long before humans evolved from their primate ancestors, they continue to coexist and coevolve with humans today, flourishing as both harmless and deadly companions. Within her literary work Deadly Companions: How Microbes Shaped Our History, microbiologist Dorothy Crawford begins with a dramatic account of severe acute respiratory syndrome (SARS), the first pandemic of the twenty-first century. Crawford travels back in time four billion years ago to the origin of microbes, recounting the evolutionary history of microbes, showing how microbes spread and cause epidemics, and revealing how coevolution yields host resistance. Furthermore, Crawford explores the intertwining history of microbes and humans, with the purpose to reveal the link between the emergence of microbes and the cultural development of man.
In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
INTRODUCTION Giardia Lamblia is a protozoan parasite. About 200 million people had infected in the world but this value just an estimate since that only 500 000 of new cases reported in a year. Previously, higher prevalence of occurance in the developing countries (20% and 30%) compare to developed countries (2% and 5%) (Wilson, 1984; Farthing, 1994).. Malaysia was considered as the developing country.
After watching this video where Michael Moss speaks about Salt, Sugar, and Fat in the American diet, my views about the food industry have changed. I never realized that the government was supporting the dairy industry. It amazed me to learn that the reason Americans are so attached to their dairy products is due to the strong relationship of the government and the dairy industry. Personally, I really enjoy dairy products.
INTRODUCTION Infection Prevention and Control (IPC) is one of the most important agents in the prevention of hospital acquired infections or what we termed nosocomial infections. IPC channels every member of the hospital, which includes, healthcare providers (HCP), patients and the hospitals perse. It is important to practice IPC commandment to every hospital as well as community. The Palestinian Ministry of Health (MOH) adopted the national IPC protocol.
Staphylococcal enterotoxins are thermostable and maintain heating to such a temperature at which staphylococci themselves are killed. The incidence is quite high. In fact, it is higher in the summer than at other times of the year. The reason most often is related to meat dishes and cakes with custard, perhaps because staphylococci, being resistant to high concentrations of protein, salt and sugar, thrive in these products without being competitive. Outbreaks are usually associated with the consumption of food with long shelf life at room temperature.
Tips For The Paleo Diet The Paleo Diet is Gluten Free, Dairy Free and Preservative Free. Referred to as the Caveman Diet or Stone Age Diet. The Paleolithic period was 10,000 years ago. The Paleolithic period is pre-agriculture and before man discovered fire. Beans, potatoes and plenty grains are inedible raw.
Escherichia Coli 0157: H7 This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge
Lactose intolerance, also known as milk intolerance, is the inability to fully digest the sugar, lactose, in milk. There are more than three million cases in the U.S. per year. As a result, they have diarrhea, gas and bloating after eating or drinking dairy products. A deficiency of lactase, an enzyme produced in your small intestine, is usually responsible for lactose intolerance. Many people claim to be lactose intolerant.
Our gastrointestinal tracts work hard to keep us healthy and happy. When gut health is compromised, we can face major health consequences. Here’s how to use good nutrition to keep your digestion humming along. “Heal the gut and you heal yourself.” – Gerard E. Mullin, MD
Using chitosan as a food preservative allows for reuse of this waste, making its use environmentally beneficial; while also reasonably cheap and cost-effective in production and usage. - Its biodegradability also allows chitosan biofilms to be beneficial to the environment. - The chitosan film has antibacterial and antifungal properties, meaning that it ultimately prolongs shelf-lives of the foods which it coats. - As chitosan film is a polysaccharide which physically coats the food product, it offers the advantage of being edible,