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Bubulcus Ibis Coromandus Research Paper

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PROCESS OF OOGENESIS IN Proechinocephalus egreti (A DIGENETIC TREMATODE) SHRIVASTAVA, 1960 Dr. Rajani Gautam Department of Zoology, Career College, Bhopal (M.P.) E-mail:drrajanigautam@gmail.com Abstract Present paper deals with the study of process of oogenesis of a digenetic trematode Proechinocephalus egreti (a digenetic trematode) of the family Echinostomatidae was collected from the intestine of an Indian cattle egret, Bubulcus ibis coromandus. A few of parasites were flattened on a clean slide under the slight pressure of a cover glass & fixed in Alcoholic Bouin,s fluid for 12 hours. Stains like Gover’s Carmine, Mayer’s Para carmine & Haemalum were used for the preparation of whole mounts for identification & the study …show more content…

A few of parasites were flattened on a clean slide under the slight pressure of a cover glass & fixed in Alcoholic Bouin,s fluid for 12 hours. Stains like Gover,s Carmine, Mayer’s Para carmine & Haemalum were used for the preparation of whole mounts for identification & the study of reproductive organs. The smears were prepared from the living material by puncturing the ovary with two fine steel needles under the stereoscopic binocular microscope. The semi fluid contents were allowed to spread over the clean slide & then the slide is inverted over the fixative without losing much time. After fixation with sublimate acetic the material is washed for 20-24 hours in running water. It is further treated with iodine in 70% alcohol to assure complete removal of the mercury. Now the blocks were prepared & sections were cut from 6-10 microns in thickness. Observations of both living & fixed material were made with Meopta & Carlzeis Zena binocular research microscope. Photomicrographs were made with the help of Meopta attachment camera. The films were developed in the fine grain developer (Eastman formula D-11) for 3-5

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