Microemulsion Lab Report

8. RESULTS AND DISCUSSION
8.1 RESULTS
The aim of this project was to prepare and evaluate the propofol Microemulsion. In this regard, Preformulation studies such as identification of drug by HPLC, compatibility between the drug and polymer, solubility of drug in various solvents were carried out by FT-IR. Results of the studies are presented in Table 8.1
Table 8.1: Identification and specifications of Propofol
TEST SPECIFICATION RESULTS
Appearance Colorless or very light yellow clear liquid Colorless clear liquid
Solubility Very slightly soluble in water, miscible with hexane and methanol COMPLIES
Identification By HPLC. The retention time of principal peak obtained in the solution chromatogram should correspond to that of principal peak

Spectroscopy:
Compatibility of the Propofol with Solutol HS 15, Ethyl alcohol, PEG 400 and Polysorbate 80 used to formulate Microemulsion was established by Infrared spectral analysis. I.R. spectral analysis of Propofol, Solutol HS 15, Ethyl alcohol, PEG 400 and Polysorbate 80 and combination were carried out to investigate the changes in chemical composition of the drug after combining it with excipients. Figure 8.2: IR Spectrum of drug Propofol

Figure 8.3: IR Spectrum of Solutol HS 15 Figure 8.4: IR Spectrum of Microemulsion
8.1.2 Analytical Methods
 Determination of λ max of Propofol:
A stock solution 1 mg/ml of propofol was prepared in Isopropyl Alcohol and scanned between 200-400 nm. The spectrum is reported in Figure.4.1 The absorption maxima of 274 nm was selected and used for further studies. Figure 8.5: λ max of propofol.
 Calibration curve of Propofol in Isopropyl Alcohol Table8.2: Standard curve plotted between concentration and absorbance.
Concentration(µg/ml) Absorbance
0 0
10 0.218
20 0.396
40 0.567
60 0.874
80 1.259
100 1.577

Figure 8.6: Calibration curve of

There is a need for selection of Polymers and excipient, which are compatible with the drug and among themselves and also physiologically safe and biocompatible. Preliminary idea about the behavior of the dosage form formulated, using the prepared and selected ingredients and their singular and collective effect on the physicochemical and pharmaceutical properties of the dosage form also needs to be generated during this