Propolis Lab Report

.1.Propolis samples

The present study was carried out using propolis from several regions of Morocco (Table 1).

These areas are different in their plants and climates.

2.2. Preparation of the ethanol extract of propolis (EEP)

Propolis samples (10 g) were cut into small pieces and extracted with 100 ml 70% ethanol at ambient temperature by macceration under agitation for a week. The hydroalcoholic extract solution was then filtered through a Whatman filter paper to eliminate the residual mass, and centrifuged for 10 min at 4000tr.

2.3.Wax Extraction and quantification

The content of wax in different propolis samples was estimated according to a procedure described by Giulia Papotti et al. 2012 with some modifications [23]. Three grams of frozen propolis was powdered and treated

Briefly, 25 µl of ethanolic extract of propolis was added to 1 ml of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate, and 4 mM ammonium molybdate). The mixture was capped and incubated in a thermal block at 95°C for 1h. The absorbance of the reaction mixture was measured at 700 nm against a blank. Ascorbic acid was used as the standard calibration. The results were expressed as milligrams of ascorbic acid equivalent per gram of sample.

2.10. Free radical scavenging activity on DPPH

The effect of DPPH radical was evaluated by the method of Kumazawa, 2004 [20]. Twenty-five µL of various concentrations of propolis samples were added to 825 µL of ethanolic solution of DPPH. Absorbance measurements were read at 517 nm, after 20 min of incubation time at room temperature (A1). Absorption of a blank sample containing the same amount of methanol and DPPH solution acted as the negative control (A0). The percentage inhibition [(A0–A1/A0)*100] was plotted against phenol content and IC50 was determined.

2.11.Scavenging activity of ABTS radical