The detergent attaches to the cell membrane and capture the protein and lipids of the cell membrane causing the cell to rupture. Then, the cell contents and DNA are released to the outside of the cell. The lysis buffer added causes the double stranded DNA in the cell to become single stranded DNA by disrupting the hydrogen bond between the bases. Next, acid is added to neutralize back the DNA to form double stranded DNA again. After centrifuge, the supernatant is collected as that is the plasmid while the pellet is the debris including protein and lipids.
Its role is to act as a medium in the cell. The cytoplasm has a large amount of organelles also known as little organs. Large protein molecules called enzymes are found in both the cytoplasm and in the organelles. Enzymes carry out many chemical reactions in order to create energy, they also transform raw materials into useful substances, or break down old proteins to be recycled. The cytosol in a cell does more than just suspend the organelles.