SIM tube was used as well as the Triple Sugar Iron (TSI), MacConkey agar (MAC) and Citrate Slant. The SIM tube is used to identify hydrogen sulfide production, indole, which is a by-product of tryptophan which is broken down by tryptophanase and motility. The streaking technique used is a half stab. The TSI has an orange color and it used to identify carbohydrate fermentation specifically glucose, lactose, and sucrose fermentation. The TSI is used to observe the slant and butt of the tube as well as to identify if gas was present and if the organism produced hydrogen sulfide.
The tube was placed back in incubation for 96 more hours to observe any more positives. 2.10 Catalase Test A trypticase soy agar plate was used and after incubation, four drops of 3% Hydrogen Peroxide was added to the plate to flow over the bacterial growth. A presence of bubbling was observed. 2.11 Starch Hydrolysis A starch agar plate was inoculated with a streak of the unknown bacteria and then incubated. On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids.
We then slowly added 25ml of chilled deionised water to the filtrate to initiate crystallization by using a measuring cylinder and a dropping pipette, once we had done this we left it for about 10 minutes to allow crystallization at room temperature. We then weighed a filter paper which we will use later in the experiment. We then collected the crystallized acetylsalicylic acid by vacuum filtration in a Buchner funnel and washed the product with a little ice-cold water. We then pre-weighed a clean, empty watch glass and labelled it with our initials and the date, we did this do we could easily identify that it was ours when we go to weigh it with the crystals on. We
Once the cola starts to boil, continue to boil it for another 10 minutes so that the carbon dioxide is removed. When the cola has finished boiling, cool it in an ice bath and pour the cola back in the volumetric flask and use distilled water to fill the flask to compensate for the evaporated water. Using a volumetric pipette, transfer 60ml of the cola to a beaker and put the magnetic stirrer in the beaker. Submerge the conductivity probe in the cola. Fill up the burette with NaOH
Rose Bengal-(bis(aminoethyl)ethylene glycol) (2) from Rose Bengal disodium salt (1) The synthesis was done following procedure from . Rose Bengal Na+ salt (915 mg, 0.90 mmol) was dissolved in DMF (2ml) and DIPEA (0.312 ml, 1.80 mmol), HATU (308 mg, 0.81 mmol) were added. After activation for 15 min, the mixture was added to O-Bis-(aminoethyl)ethylene glycol trityl resin (309 mg, 0.31 mmol) preswollen in DMF for 2 hours. The coupling reaction wrapped in aluminum foil was allowed to proceed overnight on a nitrogen bubbler apparatus. The resulting red-burgundy coloured resin was filtered and washed well with DMF.
* In the above RB, a calculated amount of 1.2 equivalent amount of PTSH was added during continous stirring. * To the RB a condenser was attached and it was put on refluxing for a time period of 18 hours at a temperature of 80 degrees in an oil bath. * Post refluxing, the condenser was removed and it was left undisturbed for 1 day and then cooled to a temperature of -20 degrees by keeping in the freezer for setting of hydrazone crystals. * Once the crystals were formed, the supernatant was decanted and the crystals were transferred to a beaker where they were washed with cold methanol and then dried. * These dried hydrazone crystals were used in the subsequent steps.
coli (Table 1) by using the well agar diffusion method. The multidrug resistant pathogens were initially propagated at 37 °C in nutrient broth. The overnight grown cultures were then again sub-cultured into nutrient broth media for 2 h till 0.01 OD. Subsequently, 100 μL of each culture was then spread uniformly onto nutrient agar plates. Wells of 6 mm diameter were made on agar plates using an agar well borer.
5-aminotetrazole monohydrate: In a 250 ml round-bottom flask equipped with a condenser for refluxing (90 °C) and a magnetic stirring bar, 5.00 g (5.95 mmol) dicyandiamide (three times crystallized), 7.47 g (11.9 mmol) sodium azide and 11.00 g (17.8 mmol) boric acid and 100 ml of water is added and allowed to reflux for 24 hours, after the completion of the reaction, until the solution pH to about 2 to 3 as hydrochloric acid 37% is added (about 12 ml) Then the reaction mixture was cooled in a refrigerator for 18 hours and the white crystals formed. The mixture was filtered and washed three times with 10 ml of water and and dried in 60 °C for 5 hours and finally 45.8 g of product by it will be obtained. 5-Aminotetrazol monohydrate: Yield:,
Ignite with a meker burner for about 1 hour. Complete the Ignition by keeping in a muffle furnace at 500 °C to 570 °C until grey ash results. Cool and filter through whatman filter paper No. 42 or its equivalent. Wash the residue with hot water until the washings are free from chlorides as tested with silver nitrate solution and return the filter paper and residue to the dish.
ABSTRACT The experiment aims to find the concentration of acetic acid in the vinegar sample by titrating it to a standardized NaOH solution, a base solution. A standardized 0.100 M NaOH solution was prepared from an available concentration of NaOH specifically a 1.00 M NaOH. The volume of the standardized solution (titrant) used which is 0.100 L was calculated using the M 1 V 1 = M 2 V 2 relationship. It was then gradually added to the vinegar solution until the acid-base reaction was completely neutralized. To regulate the reaction, phenolphthalein, an acid-base indicator, was added beforehand to the vinegar (analyte).