When the beam is subjected to cyclic loading, the graphs are shown in Figure 7. The energy absorption capacity was calculated as the area under the hysteresis loop of the load deflection diagrams. The cumulative energy absorption capacity of the beam-column joint was obtained by adding the energy absorption capacity of the joint during each cycle considered. In this study total energy absorption capacity was calculated from the each cycle Peak load verses deflection curve. 4.2 First crack and ultimate load All the specimens were initiated the crack at beam column junction after the first crack load, further increase of load the cracks started to widen and developed in upward direction of beam.
for the determination of the minimum inhibitory concentration (MIC) of the active extract. The sStarting solutions of the tested extract were obtained by dissolving it in 5% dimethyl-sulphoxide. STwo fold serial dilutions twofold dilutions of the extract were made within a concentration range from 0.04 to 40 mg/mL in sterile 96-well plates containing Mueller–Hinton broth for bacterial cultures and a Sabouraud Dextrose SD broth for fungal cultures. Resazurin solution was added as an indicator to each well. and finally, to each well fungal or bacterial suspension was added .
The setup for the cation exchange chromatography is shown in Figure 3. This was done by plugging the bottom of a burette with a small amount of glass wool. The wool was lightly packed using a thermometer. Approximately 5 mL of Dowex 50 cation exchange resin was obtained in a small beaker, and the resin was mixed with 5 mL of pH 3 citrate buffer. This mixture was poured into the burette with the stopcock closed.
After which the digestions were examined by gel electrophoresis. The samples were run on a 50 mL 0.9% (w/v) agarose gel in 1X TAE buffer at 100 V until the leading track dye traveled 2/3 the distance of the gel. The gel was then soaked in GelRed for 20 minutes and examined under UV light. To prepare the digestions 10 μL of each digestion was mixed with 2 μL of 6X track dye in a micro centrifuge tube. 12 μL of 1 kb DNA ladder and each digestion was run on the
Tube with negative results or does not indicate the presence of growth, then conducted subculture with solid growth media each bacteria as test assertion MIC value chloramphenicol preparations hydrogel. As many as 20 mL of growth medium was prepared and then given solids have zones for the variation of concentration with the method MIC macrodillution preparations showed the absence of growth. With the method of scratch, the results of the saucer MIC subculture incubated at 37 °C for 18 h. The results form bacterial colonies scratches subculture. If there were scratches (+) then showed the presence of growth, when no scratches (-) then the growth does not occur. The data obtained was made in the form of a
Using a blank cuvette place the commercial liquid and calculate the concentration and absorbency and which dyes would be in this liquid. Results: Maximum wavelength of Red Dye #3 Solution Absorbance Wavelength Max Concentration Solution 1 0.489 A.U. 526 nm 9.1 x 10-6 mol/L Solution 2 0.188 A.U. 525 nm 1.8 x 10-6 mol/L Solution 3 0.705 A.U. 525 nm 1.5 x 10-5mol/L Solution 4 0.682 A.U.
The same water was used for mixing and curing of concrete cubes. Name of Test Results Coarse Aggregate Fine Aggregate Specific gravity 2.56 2.63 Absorption (%) 0.51 0.71 Fineness Modulus 1.6 6.9 Table 3: Physical properties of aggregates Pozzolan: The cement replacement material that used in the test was local natural pozzolan from Mont Popa. The chemical composition of pozzolan is given in Table 4. It is evident that the local natural pozzolan conforms to the requirements of ASM C 618 and hence, can be used as a partial replacement of the production of roller compacted concrete. Description Composition (%) Local Natural Pozzolan Requirements as per ASTM for class N Silicon dioxide (SiO2), aluminum oxide (Al2O3) and iron oxide (Fe2O3) 77.3 Min 70.00 Sulfur trioxide (SO3) 0.34 Max 4.00 Loss on ignition (%) 2.26 Max 10.00 Table 4: Comparison of local natural pozzolan with Class N of ASTM C 618 Method: The soil compaction method is the most widely used mixture proportioning method for RCC pavements.
Table 24: Micromeritic characterization of OXB Sr. No. Parameters Result 1 Loose bulk density 0.28±0.03g/cm3 2 Tapped density 0.46±0.06g/cm3 3 Carr’s Index 37.44±0.03 % 4 Hausner’s ratio 1.59 5 Angle of Repose 26º 34’ From the above results it is evident that OXB exhibits poor flow properties. 8.1.2 Determination of solubility The solubility data for OXB as observed in 0.1 N HCl, pH 4.5 Acetate buffer, pH 6.8 Phosphate buffer and Purified water is presented in Table 25. OXB exhibited a pH independent solubility phenomenon in all pH conditions and various aqueous buffers. Table 25: Solubility data of OXB BCS solubility (pH solubility) profile of OXB Sr. No Media mg / ml 1 0.1 N HCl 249.119 2 pH 4.5 Acetate buffer 258.623 3 pH 6.8 Phosphate buffer 260.877 4 Purified water 253.200 *Average of three determinants 8.1.3 UV-VIS spectrophotometric method for OXB 184.108.40.206 Selection of
The reaction will be increased because as explained in the third paragraph, cutting the magnesium strips into smaller pieces increases the amount of collisions. It increases them because when there are more, they are more exposed to other particles and they have more to collide with. Variables: Independent: Although the length of the magnesium strips will be controlled. The way they will be inserted into the cylinder wont. In the experiment there will be three trials, in the first one the magnesium strip will be inserted as a whole piece, in the second it will be cut into smaller pieces, and in the third into even smaller pieces.
It then moves on to non-uniform plastic deformation as necking starts to occur and when it reaches the fracture stress, the aluminium alloy experiences a ductile fracture.Aluminium alloy experience ductile fracture as it has high tensile strength, which holds the metal atoms together resisting deformation, hence when it has reached its unbearable tensile stress, the aluminium alloy would then fracture. Aluminium alloy has the highest Young's modulus compare to the other 2 specimens. -40-200204060801001201401600 0.05 0.1 0.15 0.2 0.25Stress (MPa)StrainoffsetAlAlloy Polystyrene (PS) Polystyrene undergoes elastic deformation with little plastic deformation before experiencing a brittle fracture as seen on the graph. This is due the high young's modulus polystyrene has, as it has a benzene-ring side group that forms strong intermolecular van der waal's forces of interaction, however, that restricts polymer chain from rotating, hence when high amount of stress is applied to it, it would fracture, instead of sliding away to form new bonds with neighbouring atoms. -1001020304050600 0.005 0.01 0.015 0.02 0.025 0.03 0.035 0.04Stress