Abstract This report aims to summarize and highlight the potential of Push-Pull Test in evaluating the bioremediation community. A brief description on Pull-Push test, the experiments involved and the necessity for it opens up the discussion in this report. The rate of bioremediation can be estimated by the breakthrough curve which can be obtained using an analytical method on the extracted samples. The utilization of the dilution factor will also be introduced in this report. This is followed by the advantages and constraints of the Push-Pull test. After gathering all the information presented in previous sections, a case study of co-metabolism of chlorinated ethene will be elaborated to showcase the understanding of the group on the …show more content…
Biodegradation of the target contaminant is sensitive towards the environmental conditions such as nutrient, pH and temperature, which are difficult to reproduce in laboratory. Furthermore, samples collected from the field-site may not be of best depiction due to the heterogeneity of the soil (Brusseau, Piatt, Wang, & Hu, 1999). Hence, the best way for evaluation has to be done on the field-site itself which is by Push-Pull Test in the context of this report. Push pull test is useful for regulators to evaluate the soundness of a proposed or on going in-situ bioremediation system. It can also be useful to the researcher that evaluates the feasibility of utilizing an intrinsic or in-situ bioremediation of a target contaminant by evaluating the biodegradability potential of contaminants. 2. What is Push-Pull Tests? Push-pull tests are conducted by “pushing” a test solution of known concentration into the saturated zone of an aquifer via a well. The samples were then “pulled’ out of the aquifer via the same well over time to obtain information on the magnitude and rate of the biodegradation as shown in Figure 1 (Istok, Humphrey, Schroth, Hyman, & O'Reilly, 1997). Push-pull test is useful in measuring the rate of chemical or microbial reactions, which is useful in reactive transport modeling (Istok J. D., …show more content…
Among these, the third criteria is the hardest to fulfill because the organisms that are capable of degrading the contaminant in the laboratory may not be able to carry out the same task in the real
The reaction was repeated 3 times and average rate noted. From these rates a graph was plotted which describes the relationship of the pressure produced and number of drops added. The reaction rates were measured by Kpa/min and were written to 4 figures for precise results. Time was measured by stop watch. Table 4 shows a summary of all the groups which participated in the lab session.
1. Introduction The objective of the experiments was to determine the identity of an unknown compound in order to properly dispose it. The process of disposal is very important when dealing with chemicals. Some chemicals are very
ST Report In the experiment, the problem was the contaminants that were affecting the quality of the water samples. To fix this issue, three scientists had to determine the contaminants that were present in the samples. One sample was from the school sink and the second sample was from an unknown source. The scientists conducted many tests to figure out what pollutants were present in the water.
The SIM tube was first inspected for black precipitate indicating sulfur reduction and cloudiness around stab line for motility. Kovacs reagent was added in the tube a red color change in the alcohol layer. Red which also indicated is was positive for indole production. Methyl Red is used to determine if an organism is capable of performing a mixed acid fermentation. Methyl Red were added to MR test tube.
After record your data and determine the absolute rate of the enzyme-catalyzed reaction. Based on the data and observations the hypothesis was accepted. It was accepted because when pH were changed to a variety of levels the transmittance began to get higher reaction rates. The increased absorbance means greater amount of product and a higher reaction rate will be produced.
The purpose of this experiment was to analyze the effects of the variables: temperature, pH, and enzyme concentration, on the enzymatic reaction rate of catalase and the level at which its products are released, measuring the rate of absorption using the indicator solution guaiacol and a spectrophotometer to develop a hypothesis of the ideal conditions for these reactions. My hypothesis is that the extremes in concentration, temperature and pH will negatively affect the Au rate. This experiment used 11 solutions contained in cuvettes. Each cuvette, once mixed, is placed in spectrophotometer and then a reading taken every 20 seconds. Cuvettes 1, 8, and 10 are used as blanks to zero out the spectrophotometer.
Factors that affect chemical reactions Intro: this experiment is to show us what factors affect the rate of a chemical reaction. Supplies: The Works Liquid Toilet Bowl Cleaner, TUMS antiacid tablets, a non-serrated knife, a spoon, a stirring rod, four test tubes, flame heater, a small beaker for the boiled water, rubber gloves, and safety goggles. Procedure: we filled a beaker 3/4 of the way full with tap water and brought the water to a boil. We then labeled our test tubes 1,2,3,4.
2. Next, we will discuss the limitations of animal testing. 3. And finally, we will look through the existing alternatives that may limit the overall scope of the problem.
purpose the propose of this experiment was too see if the chemical reaction of a enzyme can be made faster. Hypothesis I think that a warm environment would be best to make an enzyme’s reaction faster. because a protein can move faster in heat.
The first test conducted was a Simple stain. This allowed me to be able to differentiate whether I had a Cocci or Rod shaped bacteria under the microscope. It also allowed me to know which test I showed go to next. The second procedure conducted was the Catalase test. This test was to check for the presence of the catalyst catalase which liberates molecular oxygen.
By using a spectrophotometer to measure absorbance at 420 nm, the rate of enzyme activity after all reactions have come to a stop can be
A scale of zero to five was used to describe the reactions, with zero being no reaction at all, one being a slow reaction, and five being a very fast reaction. The materials used were a test tube rack, six test tubes, a test tube clamp, forceps, a graduated cylinder, four small pieces of liver, one piece of potato, one piece of hamburger meat, approximately forty milliliters of hydrogen peroxide in a forty milliliter beaker, a splint, and matches. An ice bath and boiling water was required for testing, where a hot plate was used to boil the water. Each test tube given a label, which were “cold”, “room”, “hot”, “warm”, “potato”, “meat”, and
H20 + 2 O2 This experiment will use 1% catalase solution and 3% hydrogen peroxide solution, both diluted into water so the reaction slows down. Temperature will be controlled in this experiment to change the reaction speed of the enzyme and the substrate, this is what the experiment is looking at. The effect of the temperature will be determined by how much gas is released in two minutes, which will change the pressure inside the test tube and will be measured by a gas
The CO2 gas produced can be used as an indicator for the rate of reaction as the amount of CO2 gas that is collected with in a fixed time is proportional to the rate of reaction. Therefore, the average rate of reaction can be calculated by measuring the amount of CO2 collected for a set period of time. The rate expression of the reaction is written as: rate = k[CaCO3]a[HCl]b 1 http://www.nlm.nih.gov/medlineplus/druginfo/meds/a601032.html 2 http://www.thechemicalblog.co.uk/10-uses-of-hydrochloric-acid/ Page 2 of 7 k represents the rate constant, a and b signify the order of reaction with respect to the reactants. The order of the reaction is the power to which the concentration of that reactant is raised to, for example, doubling the concentration of a reactant that is first order would double the rate of reaction while doubling the concentration of a reactant that is in the second order would quadruple the rate of reaction.
Then, tests are performed to determine if the products of aerobic and anaerobic respiration are present in the flasks. The citric acid cycle consists of a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and chemical energy in the form of ATP (Biology). The tests detect the presence of carbon dioxide and ethanol. Carbon dioxide should be present irrespective of the type of respiration taking place, but ethanol is present only if fermentation has occurred. Another factor that can indicate whether fermentation occurred or cellular respiration occurred is the amount of glucose utilized during incubation.