1ml of pipette is used to transfer 0.1 ml of sulfanilamide solution into the test tubes and shaken gently. 7. The reagent allowed to react in 2-10 minute to complete the reaction. 8. 0.1 ml of naphthyl-ethylene diamine reagent is added and immediately mixed.
This solution was diluted with deionized water to get sample solution of 1, 3, 5, 7ppm.0.1M solution of NaOH was used to make solution basic. Adsorbent amount was kept same for all three parameters which was 0.1g. Nano Ferrites, polyaniline, PANI nanocomposits were shaken for 30 minutes respectively. After giving contact time which was 30min for Ferrites, 30min for coated PANI/CoFe2O4 and 30min for poly aniline, sample solutions were filtered and filtrate was used to investigate the removal efficiency of adsorbent. Absorbence of all sample solution was noted using uv_visible spectrophotometer.
After 30 minutes, the suspension was filtered through whatman No. 1 filter paper. Before actual titration, the 2, 6-dichlorophenolindophenol dye solution was standardized by titrating against standard ascorbic acid solution and the dye factor was calculated. 5 ml of the aliquot was taken from the filtrate and titrated against standardized
The DEAE CL-6b gel was washed twice with 0.5m Hcl, twice with 0.5m Naoh and twice with PB ph6.0 before utilization. For DEAE 1ml gel every 1ml serum was utilized. In the wake of blending for 1 hour at 200c the suspension was centrifuged at 4500g for 25 minutes. The supernatant was thought by Ammonium sulfate precipitation and pellet was broken up in refined water and the ensuing arrangement
The mixture was incubated for three min at 37ºC in water bath. The clotting time was measured by adding 0.1 ml of CaCl2 to each tube. Three types of sample were used in the FVIII assay; lysate and non lysate of FVIII-loaded RBCs in 1, 1.5, 1.10, and 1.50 dilutions and non-loaded red cells as control sample. Lysate and intact unloaded RBCs were used as a negative
The mentioned ratio of the two materials were collected in a quartz crucible. Next, de-ionized water was added into the quartz crucible containing the nickel nitrate and urea. The de-ionized water was poured until it made up 50% of the portion of the solution After that, the entire solution was placed in an oven for about 1 hour. This was done to age the mixture and the temperature was maintained at 80oC. The solution was then taken out of the oven to be put in a pre-heated muffle furnace which was maintained at 650oC.
To the double digest tube was added 12 μL double digest master mix, and 1.9 μL sterile dH2O. To the single digest tube was added 11 μL single digest master mix, and 2.9 μL sterile dH2O. To the undigested tube was added 10 μL undigested master mix, and 3.9 μL sterile dH2O. Digests were then incubated at 37 °C for one hour and then stored at -20 °C for one week. After which the digestions were examined by gel electrophoresis.
Then the wet masses were passes though 12 mesh sieve and granules were dried at 60c for 4 hours. The dried granules after blending with talc and mg stearate in a laboratory cube blender for 5 mins. Then granules were compressed into 300 mg tablet of hardness 6-7 kg/cm3 on a tablet compression machine using 10 mm biconcave shaped
The culture was grown in a shaker incubator (S1-600R, Lab Companion) for 24 hours at 30°C. Lipid production: For lipid production, 200 ml of mineral medium in glass bottle was inoculated with 10 ml of overnight culture in YPD to a final optical density at λ = 600 nm (OD600) of 0.2 and incubated on a shaker at 150 rpm and 30°C for 72 hours. Three mineral media were used (listed in materials section, Table 3). 6.2.2 Lipid droplet staining and microscopic observation The stock solution was prepared by adding 1g of Oil Red O powder to 100 ml of isopropanol, in glass bottle. Prior to use, the working solution was prepared by diluting the stock solution with Mili Q water