We then weighed a filter paper which we will use later in the experiment. We then collected the crystallized acetylsalicylic acid by vacuum filtration in a Buchner funnel and washed the product with a little ice-cold water. We then pre-weighed a clean, empty watch glass and labelled it with our initials and the date, we did this do we could easily identify that it was ours when we go to weigh it with the crystals on. We
We used 3ml of ice and 0.5 g of unknown and 1-gram sodium carbonate and 1g unknown carbonate in 10 ml H20. when we first mixed ice with unknown it melts then we used sodium carbonate and unknown carbonate it forms white precipitate. for the final test we used 1-gram sodium bicarbonate and unknown with 15 ml of H20 then it bubbles up. After we done with all other test, we like to see the PH of the sodium bicarbonate and unknown, its initial temperature is 20 and the final temperature was 24 and then PH paper turn blue and its has the PH of 8-9 and its very
A scale of zero to five was used to describe the reactions, with zero being no reaction at all, one being a slow reaction, and five being a very fast reaction. The materials used were a test tube rack, six test tubes, a test tube clamp, forceps, a graduated cylinder, four small pieces of liver, one piece of potato, one piece of hamburger meat, approximately forty milliliters of hydrogen peroxide in a forty milliliter beaker, a splint, and matches. An ice bath and boiling water was required for testing, where a hot plate was used to boil the water. Each test tube given a label, which were “cold”, “room”, “hot”, “warm”, “potato”, “meat”, and
Fractional distillation is a separation technique used to separate two liquids with different boiling points and keep the liquid. To do this, we set it up just like the distillation lab with the 10-15mL in the test tube over the fire and the tube leading the the test tube in the beaker. The first time you go through, the same test tube is left in the whole time but you must record the temperature around every 10-15 seconds using your labquest. You then find two places where the temperature is consistent for a few seconds, this is your plateau. The second time you go through, change out the test tubes as soon as you get to your first plateau, this liquid is liquid one.
Animals were also weight immediately prior to sacrifice (fasted body weight). Animals were sacrificed under anesthesia with diethyl ether, and then blood samples were immediately collected in clean and dried Wiesserman tubes from the portal vein. First part of blood was collected in tubes containing potassium oxalate and sodium fluoride for the estimation of plasma glucose by O-toluidine method of Sasaki et al. (1972). Second part of blood was left to coagulate then centrifuged at 3000 rpm for 15 minutes to obtain serum to estimate some biochemical parameters.
After the evaluation of stomach for ulcers, the gastric mucosa of glandular portion was scrapped with the help of two glass slides, weighed (100 mg) and homogenized in 1 mL of a 0.15 M, ice cold potassium chloride (KCl) solution and centrifuged at 3,000 RPM for 10 minutes (REMI centrifuge). 1 mL of suspension was taken from the above tissue homogenate in test tube and 0.5 mL of 30% w/v TCA (trichloroacetic acid) was added to it, followed by 0.5 mL of 0.8% w/v TBA (thiobarbituric acid) reagent. The tubes were then covered with aluminium foil and kept in water bath for 30 minutes at 80 °C. After 30 minutes, tubes were taken out and kept in ice-cold water for 30 minutes and centrifuged at 3000 rpm for 15 minutes (R-BC DX REMI centrifuge). The absorbance of the supernatant was read in spectrophotometer (UV-1601, SHIMADZU) at 540 nm against blank.
Experiment and Results Sample 100 ng/µl DNA was extracted from the cricket Acheta domesticus using the phenol-chloroform methods described in Davies et al., 2012 , dissolved in Tris-HCl-EDTA (TE) buffer and kept frozen at -20˚C. In initial tests, portions of the extracted DNA were suspended at the same DNA concentration as the control sample in solutions of magnesium chloride, magnesium sulfate, ammonium sulfate, lithium chloride, and nickel chloride. Each salt was mixed in three different concentrations, including 100 mM, 10 mM, and 0.1 mM. Then DNA in each salt concentration was incubated at different temperatures: 25˚C, 42˚C, 65˚C, and 95˚C, for fifteen minutes. The products were then loaded onto an agarose gel and allowed to run in
Radioactively Labeled Azole Import by M. oryzae Radioactively labeled FLC (3H-FLC), (481 GBa/mmol, 13 Ci/mmol, 1 µCi/µL; 77 µM FLC) was custom synthesized by Amersham Biosciences. The drug concentration used during the import assay was well below the Minimum Inhibitory Concentration (MIC) for the strain (M. oryzae FLC MIC >32 µg/ml). To directly measure azole intracellular accumulation in the fungal cell, we used 3H-FLC in our drug uptake assay designed for M. oryzae.
Lastly, the third graduated cylinder was labeled ‘V’ since it contained 440g of ascorbic acid also known as vitamin C. All three graduated cylinders were put in the 70℃ water bath after adding their own catalase alongside three test tubes of H2O2 for 30 seconds. After the specified period of time, the graduated cylinders were taken out of the bath and the H2O2 was added to each of them and that is when the timer started. The recordings were taken every 30 seconds for 90
After we set the spectrophotometer to zero, we mixed the second enzyme with the second substrate and promptly poured it into a clean cuvette to then be put in the spectrophotometer and record the absorbance at zero seconds, and again every thirty second for three
Repeat steps 13-16 for two more trials to achieve precise data. Take your beaker of water (150 mL) or ice out of the freezer. Measure 50 mL of water and see if it is 10 degrees celsius (if the water’s temperature is more than 10 degrees celsius, leave it in the freezer longer. If the water is colder than 10 degrees celsius, leave it out to warm up).
Research Protocol – Monitoring of the Daphnia magna heart rate The experiments focused on the four treatments of nicotine, caffeine, ethanol, and double distilled water (placebo). 180 μL of each bioactive solution (nicotine was covered with foil, due to light sensitivity) and 120 μL of double distilled water were placed into labeled eppendorf tubes to dismiss cross-contamination, and were placed on ice to match the environment of the Daphnia to reduce any added stress on the Daphnia. One Daphnia was placed on the testing petri dish, and then all the excess pond water was removed with a transfer pipette.
The dried roots of Inula racemosa were pulverized and sieved with 100 ~ 200 mesh. The herb powder was placed into a glass bottle. Ultrasound-assisted extraction was carried out in an ultrasonic cleaner RK102H (Bandelin sonorex, Germany). The powder of Inula racemosa was extracted three times under the following conditions: the ratio of material to solvent was 10:1, undergoing ultrasonic treatment 30 minutes at 25 °C, 100 kHz /450 W.31 Before large extraction, a small-scale extraction experiments were carried out: 95% ethanol and ethyl acetate as the extractive solutions was investigated, respectively.
5. 150 ml of the solution in beaker A was added to the separating funnel with 10ml of chloroform. The funnel was gently shaken and vented to release the pressure. This was done five times. 6.
First, label one micro centrifuge tube +pGLO and another –pGLO. Using a sterile transfer pipet, transfer 250µl of competent cells (E. coli + CaCl2) into each tube and place them in crushed ice. Examine the pGLO plasmid DNA solution with the UV light and note your observations. Pipet 10µl of pGLO plasmid into the +pGLO tube and mix, close and return it to the ice rack. Do NOT add plasmid DNA to the –pGLO tube.