The retention factor for each dye was calculated. F or each of the Kool-Aid flavors, 2.0 g was weighed out from the packet and 5mL of water was mixed in with them each. mL of 0.1% NaCl solution was added to 100mL of bottled water. The six chromatography strips
As it was done in the Experiment A, 20 drops of 0.2 M acetic acid and 10 drops of 2% starch solution was mixed well with the juice solution. Before adding the iodine solution, the initial reading of the burette was taken. Then, the titration was started using the iodine solution into the burette with continuous swirling of the flask slowly and carefully. Once the color change started to appear, titration was stopped and final burette reading was recorded. Finally, the amount of vitamin C in the mandarin orange was calculated by using the standardization factor and used iodine solution.
Standard solution The standard solution was tested for its value and was used in the calculation of the patient's urea and creatinine value. ii. Running patient's specimen a. Creatinine 1ml of creatinine reagent 1 and 200ml of creatinine reagent 2 was pipetted into the same 1ml cuvette. Following that, 40ml of patient's sample was transferred into the cuvette. The absorbance of the mixture was measured at 30s (A1), 5min and 6min intervals.
SAMPLE REQUIREMENT: Type of specimen: Serum (Blood collected in Plain tube and centrifuged to separate serum). PRINCIPLE OF TEST: In the presence of a strong base such as NaOH, picrate reacts with creatinine to form a red chromophore. The rate of increasing absorbance at 510nm due to the formation of this chromophore is directly proportional to the creatinine concentration in the sample and is measured using a Bichromatic (510,600nm) rate technique. To correct for non-specific reaction caused by serum/plasma pseudo-creatinine chromogens, including proteins and ketones, the results for serum or plasma are corrected by -18 μmol/L (-0.2 mg/dL). (2, 4, 14-18) Alkaline pH (NaOH) Creatinine + picrate red chromophore (absorbs at 510nm) EQUIPMENTS & APPARATUS: Siemens Dimension® clinical chemistry analyzer (X pand and RxL Max) Flex reagent cartridge, Assay Cups, tips, pipettes.
Referring to Table 1, the reactants for each run were transferred to an Erlenmeyer Flask (250 mL) via a buret. Using a precision pipette, the volume of I3- required for each run was carefully extracted and poured into the flask containing all of the reactants. Immediately after the Iodine solution was placed in the flask, the LabQuest began collection data. Meanwhile, a small portion of the solution, was used to rinse the cuvette, then using a disposable pipette a small amount of the solution was transferred to the cuvette (approx. ¾).
A well known example of this plant is the coffee bean. Thus, to calculate the caffeine content of soft drinks, we may use the process of HPLC. And as we define HPLC (High performance liquid chromatography) and formerly known as high pressure liquid chromatography, is a classical quantitative analysis method by which we use pump in a pressurized liquid to extract the mixture and get the sample of caffeine content of soft drinks. Through this method we will able to analyze the caffeine contain. There are disadvantage and advantage of having caffeine in soft drinks by ingesting it.
H and S were diluted 20x and P1 and P2 were diluted 2x to make up a total volume of 1ml each. 50ul of each diluted sample was pipetted into 8 wells of the microplate and 50ul of each protein standard was pipetted into 2 wells. 50ul were incubated with 50ul of alkaline copper reagent. 50ul of alkaline copper reagent was added to every well containing water, buffer, sample or standard and was incubated for 30 minutes. 100ul of Folin reagent was added to the wells and incubated for another 20 minutes.
In a typical process, appropriate amounts of SO dye and the catalysts were first mixed in a small amount of distilled water. Then, a certain volume of NaBH4 solution was introduced into the above system to form a 3 mL solution. Here, the concentrations of the SO dye, NaBH4 and the catalyst were 2×10-5 mol L-1, 40 mmol L-1 and 200 mg L-1, respectively. Firstly, the catalytic degradation of SO dye in the absence of Bi2S3 NSs by NaBH4 was studied. A small amount of SO dye was degraded.
The concentration of ethanoic acid in the vinegar can be determined through stoichiometric calculations, Using the values obtained from the titration, and also the chemical equation as a reference. Phenolphthalein indicator is used in this acid-base titration Equipment and materials: Commercial vinegar, Yamaha brand 0.1 mol/dm3, NaOH soloution Phenolpthalein indicator soloution (50.00 ± 0.5 cm3 ) cm3 burrete (250.00 ± 0.5 cm3) volumetric flask a (250 cm3± 0.5 cm3)
Burette can actually transfers water more accurate compare to pipette and micropipette. The burette calibration were done using 5ml, 10ml, 15ml, 20ml and 25ml of water. Each volume of water was repeated three times to obtain a more accurate result. When the experiment is conducted, the mass of the empty Erlenmeyer flask is taken before each trial. For the nominal water volume of 5ml, apparent mass of water for Trial 1, Trial 2 and Trial 3 are 5.064g, 4.976g and 4.945g respectively.