ipl-logo

Rettgeri

872 Words4 Pages

The first task that I had to do upon receiving my unknown was making two subcultures of it. The “stock” culture would serve as my emergency backup in case my bug got contaminated, while the “working” culture was the one I would utilize to perform the biochemical tests. The next thing I did after this was the gram stain. The purpose of this was to find my bug’s gram designation and morphology so that I would be working in the right direction. My bug retained the safranin stain which meant it was gram negative. The cells of the bacteria were rod shaped, therefore, their morphology was bacillus. The following test I did was the Oxidase test. The unknown tested negative for this particular test because the oxistrip failed to turn blue shortly …show more content…

rettgeri is a gram negative bacillus that is part of the genus Providencia and a member of the family Enterobacteriaceae. P. rettgeri and the other species in this genus are found in animal reservoirs, soil, water, and sewage (Charbek). The species in this genus are also responsible for human infections in the urinary tract, and they are also associated with gastroenteritis and bacteremia (Charbek). The first species in this genus was isolated by Rettger in 1904 when it was initially found in chickens in what was thought to be an epidemic in fowl cholera (Charbek). Since their discovery, species in this genus have undergone numerous taxonomic changes because they are closely related to species from the genera Proteus and …show more content…

rettgeri has been isolated in crocodiles with meningitis/septicemia, and in chickens with enteritis. Human isolates of P. rettgeri have been found in the blood, urine, stool, throat, perineum, axilla, and in wound specimens (O’hara). A study conducted in 2001 concluded that P. rettgeri and its counterparts were found in 18% of patients with complicated urinary tract infections (Charbek). It has also been reported that one specific strain of P. rettgeri caused the death of at least one individual who had a urinary tract infection (O’hara). P. rettgeri is also capable of causing nosocomial infections. In 1976, an outbreak of this bacteria affected 127 patients over a 22 month period in a hospital in Tennessee (O’hara). In addition, P. rettgeri has been known to cause ocular infections such as keratitis, conjunctivitis, and

Show More
Related

  • Unknown Microbial # 398

    532 Words  | 3 Pages

    Methods Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398. The identification of unknown #398 followed test procedures from Brown1.

    Read More

  • Unknown Culture Lab Report

    1054 Words  | 5 Pages

    Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.

    Read More

  • Unknown Bacteria Lab Report

    1200 Words  | 5 Pages

    The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.

    Read More

  • Lab Report Identifying Unknown Microorganisms

    1596 Words  | 7 Pages

    In this experiment, we cultivated an unknown specimen containing two microorganisms. The purpose of this experiment was to use a variety of biochemical test previously learned in the lab to identify the unknown bacteria. The identification of unknown bacteria is a major part of microbiology. Microbiologist observe samples such as blood and sputum in the laboratory for the presence of microorganisms. Identifying unknown bacteria is extremely important in clinical settings because it helps physicians find treatment for infections.

    Read More

  • Escherichia Colo Lab Report

    308 Words  | 2 Pages

    This lab used Escherichia coli (E. coli) bacteria (Kok , 19840). This is because Escherichia coli can be simply grown in Luria broth or on agar, and also has a comparatively small genome of five million base pairs.

    Read More

  • Unknown Lab Report

    1444 Words  | 6 Pages

    These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where. A number of tests can be ran on an unknown bacteria to determine their ideal

    Read More

  • Unknown Identification Lab Report

    568 Words  | 3 Pages

    The Unknown Identification Lab was an experiment that provided the opportunity to apply all the tests that were learned in the semester of lab, to identify the two bacterias that remain unknown. Gram- staining and two other tests will be used to identify the unknowns. This experiment is crucial to the understanding of each test, and can benefit in the ability to identify the characteristics of specific bacteria. Having a clearer understanding of the bacteria can further the research of bacteria for medicine, such as antibiotics. The understanding can also help the development of research in the environment.

    Read More

  • Staphylococcus Epidermidis Essay

    771 Words  | 4 Pages

    My bacterium turned out to be gram positive. When conducting these tests, I only had to do the coagulase test and the catalase test because when doing the catalase test, the reaction was that it had bubbled. If it did not bubble, or have a positive reaction, then I would not have had to do the coagulase test. Also, since my bacterium caused a positive catalase test, I only had to do the coagulase test and no other tests. This is because with staphylococcus organisms, these are the only tests

    Read More

  • Unknown Bacteria

    557 Words  | 3 Pages

    In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.

    Read More

  • What Causes Lyme Disease

    762 Words  | 4 Pages

    The bacteria stated prior, Borrelia Burgdorferi, is the cause of the disease and the stem of the problem. The bacteria cannot survive alone for longer than a few hours so it finds a “host” to survive inside of (MD). The most common hosts are ticks, mice, and other small mammals (MD). The bacteria has a reproduction cycle of 28-30 days, almost identical to the moon cycle (TOL). The bacteria has been known to align itself with the moon cycle, causing its victims to have noticeably worsening symptoms during full moons cycle.

    Read More

  • Unknown # 10 Essay

    650 Words  | 3 Pages

    Unknown #10 produced no identifiable macroscopic characteristics as a broth, so the first step was to Gram stain a loopful to determine the microscopic characteristics. Gram staining not only helped identify Unknown #10’s microscopic morphology but it also helped ensure the specimen was a pure culture—no other bacteria were visible when Unknown #10 was Gram stained and observed under the microscope. Unknown #10’s key microscopic morphology was that it was a very small, Gram negative bacillus. Though bacilli can possibly form endospores, no empty white centers were visible which suggested that Unknown #10 was not an endospore forming bacteria. No quick endospore stain was performed to validate this assumption since only one assigned organism was endospore forming and unlike Unknown #10, that organism was Gram positive.

    Read More

  • The Black Plague

    554 Words  | 3 Pages

    In special cases, Yersinia Pestis bacteria would get into the skin if a human had a

    Read More

  • Exercise 14 Unknown Identification Lab Report

    449 Words  | 2 Pages

    The second ½ of the organism was used for gram staining. The gram stain method was performed on the unknown organism per lab manual page 42 and two gram stain reactions were identified. Organism B was gram positive cocci in grape like clusters. Because organism B was positive I could eliminate Escherichia coli, Enterobacter aerogenes and Proteus vulgaris because these bacteria would be rod shapes. Organism A was gram negative pink rod shaped and because of gram positive morphology I could eliminate Staphylococcus aureus, Streptococcus lactis and Bacillus subtilis.

    Read More

  • Blood Agar Lab Report

    1057 Words  | 5 Pages

    After a gram stain was done unknown #257 was identified as a gram positive organism because when observed under the microscope the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with a heavy quadrant one.

    Read More

  • Staphylococcus Epidermidis Research Paper

    452 Words  | 2 Pages

    After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster. However, during decolorizing process, I put too much alcohol to the crystal violet-iodine complex making the color overly removed. That led to the result of my gram positive has slightly redish

    Read More

More about Rettgeri

Open Document