These characteristics allow us to produce drugs, agroproducts and fine chemicals. STRUCTURE The lipases are found in nature. There are many types of lipases. In common most of the lipases are having an alpha or beta hydrolase folding addition to it they also have chymotrypsin like catalytic activity. PHYSIOLOGICAL DISTRIBUTION: These lipase enzymes have a widespread biological activity in biological processes from usual metabolism of dietary triglycerides to signalling pathways and inflammatory mechanisms.
(11) References: (A) https://en.m.wikipedia.org/wiki/Citric_acid (B) https://pubchem.ncbi.nlm.nih.gov/compound/311 (4) Component Name: FD&C yellow no. 10; C18H9NNa2O8S2; 477.37 g/mol (5) Systematic Name: A) Acid Yellow 3; B) Quinoline yellow WS; and C) Food Yellow 13. (6) Structural Formula: General Description: (7) FD&C yellow no. 10 is a compound since it contains of more than one element in its molecular formula. (8) FD&C yellow no.
Magnesium is a chemical element with the symbol “Mg”. Magnesium is a shiny gray solid. The atomic number is 12 and it’s atomic mass is 24.305. Joseph Black a Scottish physician and chemist recognized magnesium as an element in 1755 but it was isolated by Sir Humphry Davy in 1808 (Periodic Table). High magnesium can be found in foods including nuts, fish, beans, avocados, yogurt, bananas, dried fruit, dark chocolate, and more.
Determination of methotrexate and its metabolites: 1. Determination methods developed till 2000: In this era the methotrexate was being used only as anti tumor agent to treat acute leukaemia, osteosarcoma, psoriasis, sarcoidosis and wegener’s granulomatosis. Due to its toxic side effects such as skin rash, myelo suppression, renal damage and mucosal ulceration the biological fluid level of methotrexate came into existence. The side effects was only when this drug was used in higher concentration. The biological fluids used were serum, urine and cerebrospinal fluid.
The great variety of species over which these compounds are found has grown as detection techniques have improved. Tannins are high molecular weight, water-soluble polyphenols that form reversible complexes with proteins through pH-dependent hydrogen bonding and hydrophobic interactions. Hydrolysable tannins (HT) contain a carbohydrate core esterified with gallic or hexa-hydroxyl-diphenic acids. Binding of HT to abomasal mucosal proteins causes lesions that result in diarrhea or constipation. Hydrolytic products of HT are absorbed from the small intestine; disrupt liver and kidney function, and cause photosensitization and dehydration.
Alkaline proteinase is used extensively as detergent additives and α-amylase are used in the starch industry (Tomazee and Klibanov, 1988). Similarly, the enzyme glucose isomerase produced using B. coagulan is used to convert glucose to fructose in the production of high fructose corn
SOD activity was calculated in terms of units/mg protein. Total protein thiols (TPT) - This assay is based on the principle of formation of relatively stable yellow color by sulphydryl groups with DTNB (Moron et al., 1979). Briefly, 0.2 ml of liver homogenate was mixed with phosphate buffer (pH 8.0), 40 µl of 10 mM DTNB and 3.16 ml of methanol. This mixture was incubated for 10 min and the absorbance was measured at 412 nm against appropriate blanks. The total thiol content was calculated by using ε=13.6x10 cm-1 M-1 Sedlak & Lindsy, 1978).
Eugenol at the concentrations of 0.2 -20 µm is suggested to be able to produce a dose dependent and reversible vasodilator response that are partially dependent on the endothelium (24). EUG has also found to have a preventive effect on dopamine depression and lipid peroxidation, which can protect depression induced by 6-hydroxyl dopamine (OHDA). Eugenol has prevented depression by decreasing lipid peroxidation and stimulating reduced glutathione (GSH) may lead to a protecting effect (25). TOXICITY of EUGENOL Eugenol is considered safe as a food additive but due to the wide range of different applications and also the extensive use and availability of clove oil; there is a great concern about its toxicity in recent years. Results of a case study indicated that ingestion of 5-10 ml of clove oil to a two-year boy resulted in distressing and crying.
The taste, smell antimicrobial properties of these substances are derived from compounds known as phytochemicals (secondary metabolites) present in the substance that inhibit growth of bacteria (Avato et al. 2002). The presence of a benzene functional group and sulfur may be an important component of the antimicrobial mechanism (Agabalogun, L et al). Presently, approximately 80% of the world population relies on botanical derived medicines as they are considered safe, free of side effects, effective against bacterial ailments and do not pose threat of bacterial resistance (Hora and Nair.1944). In this experiment, the antimicrobial properties of the powdered forms of three widely used Indian spices: Turmeric (Curcuma longa), Indian Lilac/Neem (Azadirachta indica), Ginger (Zinhiber officinale), Asafetida (Gumm oleoresin) was further investigated.
Isoniazid ( isonicotinic acid hydrazide ,INH , is a pro- drug that requires mycobacterium tuberculosis catalase peroxidase enzyme to be active against mycobacterium and does not have any effect on mycobacteria before this enzyme activation. It is one of the most commonly used drugs in the treatment of tuberculosis, because mycobacterium tuberculosis show high sensitivity to it. However it should be noted that this drug is effective against growing basil and cannot eradicate the latent basil. Furthermore, its activity against mycobacterium is carried out under completely aerobic conditions and under anaerobic conditions does not have an inhibitory effect on mycobacterium tuberculosis. The main reason for this event is the need of oxygen for