Gram-Negative Anaerobic Bacteria
Chapter VII
ANTIMICROBIAL PROPERTY
Primary root canal infections are polymicrobial, typically dominated by obligatory anaerobic bacteria. The most commonly isolated microorganisms before root canal treatment include Gram-negative anaerobic rods, Gram-positive anaerobic cocci, Gram-positive anaerobic and facultative rods, Lactobacillus species and Gram-positive facultative Streptococcus species.83 The obligate anaerobes are rather easily eradicated during root canal treatment. In contrast, facultative bacteria such as non-mutans Streptococci, Enterococci, and Lactobacilli, once established, are more likely to stay alive even after chemo-mechanical instrumentation and root canal medication.84 In particular Enterococcus faecalis has gained attention
…show more content…
Enterococci can survive in monoculture, but cause only minor lesions. The outer membrane of Gram-negative bacteria contains endotoxin, which is present in all necrotic teeth with periapical lesions, and is able to trigger an inflammatory response even in the absence of viable bacteria. Furthermore, the levels of endotoxin in necrotic root canals are positively correlated to clinical symptoms such as spontaneous pain and tenderness to percussion.85 Virulent Gram-negative anaerobic rods rely on the presence of other bacteria in their environment to survive and establish their full pathogenic potential. Such collections of microorganisms in an extracellular polysaccharide matrix associated with a surface (the inner root canal wall) are called biofilms. There is convincing evidence that microorganisms organized in this manner are far less susceptible to antimicrobial agents than their planktonic counterparts, which have been used by tradition to check the antimicrobial efficacy of substances in …show more content…
Results demonstrated that all substances showed bactericidal effect on all studied microorganisms at all concentrations and after a short contact period.90
Sen B H et al (1999) assessed the antifungal properties of 1% NaOCl, 5% NaOCl, and 0.12% chlorhexidine against Candida albicans using cylindrical dentine tubes and found C. albicans to be more resistant in the presence of smear layer than in the absence of smear layer. When smear layer was absent, NaOCl started to display antifungal activity after 30 minutes
Unknown Culture Lab Report
1054 Words | 5 PagesIdentification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
Unknown Bacteria Lab Report
1200 Words | 5 PagesThe purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
Hank Harum Case Summary
759 Words | 4 PagesAbscesses occur for a variety of reasons, cause extreme pain, and can be treated with much success. If left untreated, however, bacteria can spread into other areas of the mouth, causing serious medical conditions. Periodontal disease can cause the formation of an abscess, which is more common in cats that have a tendency to bite or chew frequently. If left untreated, facial or mouth traumas, bacterial infections, and diabetes can all contribute to the formation of an abscess. Symptoms of tooth root abscess are the following: - Bad breath - Loose teeth - Facial swelling - A
Root Canal Procedures
484 Words | 2 PagesIf you are going to have a root canal this summer, the last thing you want to do is be in pain for multiple days when it is so nice outside. Here are three key tips that will help ensure that you recover successfully and quickly from your root canal procedure this summer. #1 Take All Of Your Antibiotics After your root canal procedure, your doctor is going most likely going to prescribe a round of antibiotics for you. You will be prescribed antibiotics because the tooth you had removed was infected.
Nashua Ashut Research Paper
1581 Words | 7 PagesTooth Extraction Procedures in Nashua, NH Have you recently been told you need an extraction? Are you nervous and not sure what that may entail? We can help. We have provided some valuable information on why you might need an extraction, what happens during the procedure, and how we can keep you comfortable. Please feel free to contact our office if you have any additional concerns.
Unknown Bacteria
557 Words | 3 PagesOf the Enterobacteriaceae family, there are genera that are in the normal human flora. Some species such as K. pneumoniae and E. coli are opportunistic pathogens which can capitalize on weakened host defenses and cause food poisoning (Baron, 1996). S. enterica secrete proteins that help aid in intracellular invasion and proliferation (Hensel, 2009). K. pneumoniae is a part of the normal human mouth, skin, and intestine flora, but can wreak havoc if inhaled (Ryan,
E. Coli Bacteria
437 Words | 2 PagesAlthough the nature of this kind experiment was unable to provide us with numerical data, our graph was constructed using the average percent of E. Coli killed for each pair of cultures. To determine these percents for each substance we used the process of elimination. We put the two petri dishes side by side and used a checklist ranging from 1-100% to narrow down the possibilities. After obtaining a small range of possible percents, we made an estimate to determine the percent. After doing this for both the of cultures for that pair we averaged the two numbers.
E. Coli Research Paper
1004 Words | 5 PagesEscherichia Coli 0157: H7 This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge
Dog Saliva Hypothesis
3687 Words | 15 PagesHypothesis: It is hypothesised that dog saliva will be effective in killing bacteria. Aims: To prove that dog saliva has the ability to kill bacteria. To find possible uses for dog saliva as a disinfectant. To compare the effectiveness of dog saliva as a disinfectant to commercial
Blood Agar Lab Report
1057 Words | 5 PagesThis is where the antibacterial disc bacitracin was used to determine the bacteria’s susceptibility.
Irrigant Analysis
798 Words | 4 Pages17.5% endodontists and 18.6% non-endodontists opted chlorhexidine as the irrigant with the best antimicrobial action. It has been suggested that chlorhexidine is sporostatic but not sporicidal 13, and is said to have a disadvantage of not dissolving organic tissue, and therefore cannot be considered as the irrigant with best antimicrobial action. Only 23.3% non-endodontists agree that sodium hypochlorite is the inevitable irrigant during root canal
Kirby Bauer Diffusion Test Lab Report
624 Words | 3 PagesAseptic technique was initiated at the beginning of this experiment by cleaning the work surface with disinfected wipes. Personal protectives equipment was also worn. The material utilized in this experiment was: S. epidermidis culture broth, sterile cotton swab, streak plate, forceps in 70% alcohol, a lit tea light, and the three antibiotic disks (novobiocin, gentamicin, penicillin). The first step, I divided a plate into three quadrants and labelled them with the different antibiotic names. Using the lit tea light, like a bursen burner, I flamed the mouth of the S. epidermidis culture.
Mungbea Case Study
923 Words | 4 PagesTreatment with fungicides checked the disease severity (P≤0.05, Figure 1). Highest significant reduction in the root-rot severity index was observed with carbendazim (44.4%). Next in effectiveness was captan which significantly reduced the disease by 37.8%. Thiram were moderate in effectiveness causing 33.3% root-rot reduction. Vitavax and mancozeb were found least effective in checking the disease and recorded only 28.9 and 26.7% suppression over control.
Renal Failure
971 Words | 4 PagesAlthough OC is a superficial oral infectious lesion that is clinically detectable, it should be taken seriously due to its high potential to develop into a systemic infection in immune-compromised patients. Oral presentations of candidiasis vary from the large white plaques of pseudomembraneous candidiasis on the tongue and buccal mucosa to the palatal erythematous lesions of chronic atrophic candidiasis, and to angular cheilitis on the labial commissures .The primary etiological agent of oral candidiasis is the yeast C. albicans; however, other species that cause disease less commonly include C. tropicalis, C. glabrata, C. krusei, C parapsilosis, C. guilliermondii, and C. dubliniensis
Biochemical Test Lab Report
1371 Words | 6 PagesApproximately 60-90% of the Gram-positive bacterial cell wall is made up of peptidoglycan and interwoven teichoic acid, while only
More about Gram-Negative Anaerobic Bacteria
Unknown Culture Lab Report
1054 Words | 5 PagesUnknown Bacteria Lab Report
1200 Words | 5 PagesHank Harum Case Summary
759 Words | 4 PagesRoot Canal Procedures
484 Words | 2 PagesNashua Ashut Research Paper
1581 Words | 7 PagesUnknown Bacteria
557 Words | 3 PagesE. Coli Bacteria
437 Words | 2 PagesE. Coli Research Paper
1004 Words | 5 PagesDog Saliva Hypothesis
3687 Words | 15 PagesBlood Agar Lab Report
1057 Words | 5 PagesIrrigant Analysis
798 Words | 4 PagesKirby Bauer Diffusion Test Lab Report
624 Words | 3 PagesMungbea Case Study
923 Words | 4 PagesRenal Failure
971 Words | 4 PagesBiochemical Test Lab Report
1371 Words | 6 Pages