Rp-Hplc Essay

A NOVEL DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF NINE WATER SOLUBLE VITAMINS A new simple, sensitive, reproducible, robust and rapid high performance liquid chromatographic method developed and validated for simultaneous determination of all nine water soluble vitamins (B1, B2, B3, B5, B6, B7, B9, B12 and C) in vitamin premix. The analysis was carried out on Phenomenex C18 Luna column (250 x 4.6 mm, 5 μm particle size) using water as diluent and simple gradient mobile phase composed of 25 mM sodium dihydrogen orthophosphate dihydrate in purified water pH adjusted with ortho phosphoric acid to 2.5 and 950 ml of methanol with 50ml of phosphate buffer. The mobile phase was pumped at flow rate of 1.0 ml/minute

They are widely distributed in natural food sources and can be easily introduced into the diets to satisfy daily needs. Though vitamins are a group of organic compounds that have different structural and chemical properties, they can be conveniently categorized into two groups based on their solubility: fat-soluble vitamins and water-soluble vitamins. Fat soluble vitamins include A, D, E, and K where as the latter is composed of vitamin C and 8 B-vitamins, namely thiamin (vitamin B1), riboflavin (vitamin B2), niacin (vitamin B3), pyridoxine (vitamin B6), pantothenic acid (vitamin B5), biotin (vitamin B7), folic acid (vitamin B9) and cyanocobalamin (vitamin B12). Water soluble vitamins are readily excreted in urine without appreciable storage, so frequent consumption becomes necessary. They are generally nontoxic when present in excess of needs, although symptoms may be reported in people taking mega doses of niacin, vitamin C, or pyridoxine (vitamin B6). All water soluble vitamins function as coenzymes or cofactors, assisting in the activity of important enzymes and allowing energy-producing reactions to proceed normally. As a result, any lack of water-soluble vitamins mostly affects growing or rapidly metabolizing tissues such as skin, blood, the digestive tract, and the nervous

separated 9 water soluble vitamins with LC-MS in premix (reference no-review article no) which is expensive when compared to our developed HPLC method. The aim of this study was to develop and validate fast, sensitive and robust HPLC method for the analysis of all nine water soluble vitamins ((B1, B2, B3, B5, B6, B7, B9, B12 and C), with experimental conditions that ensure its maximum stability, and the utilization of this method for water soluble vitamins analysis to a variety of commercial products whose concentration range is low including multivitamins and various soft