The hydroquinone metabolite purified from B. methylotrophicus MHC10 was evaluated for its antibacterial activity against a panel of several Gram-positive and Gram-negative bacterial pathogens. The zone of inhibition was used to evaluate the antagonistic activity of the metabolite. The standard antibiotics Ampicillin and Gentamycin were used as the positive control. Both antibiotics showed high antagonistic activity against all test pathogens. But in the case of P. aeruginosa, the hydroquinone treatment showed little high zone of inhibition than ampicillin Lee et al.,  studied the antimicrobial activities of the purified prodigiosin and cycloprodigiosin against B. subtilis KCTC 1914, E. coli KCTC 1924, Salmonella typhimurium KCTC 1926, S.
This test is based on removing of iron from chrome azurol S (CAS) by producing of sidrophore. Five microliters of each fresh culture was inoculated onto a plate, which was then inocubated at 28°C for 72 h. The presence of an orange halo (A change in a color of CAS from blue to orange) around a colony indicated a positive result (Schwyn and Neilands, 1987). 3.1.3 Indole-3-acetic acid production IAA production was assessed in tryptone broth medium ( tryptone 10.0 g, sodium chloride 5.0 g in 1000 ml distilled water and final pH 7.2). The medium was dispensed into tubes and autoclaved at121°C for 15 min. Bacterial isolates cultured for 24h were inoculated into tubes, which containing 5 ml tryptone broth and incubated at 37°C for 7days.
Majority of the gram negative isolates were sensitive to imipenem and this was found to be in agreement with Ranjan et al.,201026. Postoperative wound infection in any hospital depends on the hospital environment and use of antibiotics. Antibiotic resistance can be controlled by appropriate antimicrobial prescription, prudent infection control, new treatment alternatives and continued
The active fractions were pooled and extensively dialyzed against distilled water. • The active elutes with highest peaks were tested for antimicrobial activity and stored at -20°C until required for further use and considered to be purified protein. (Experiment was out sourced from Biotech Company and help of Dr Rakesh is duly acknowledged.) 3.9.4 Estimation of protein content The protein concentrations of crude, ammonium sulfate precipitate and purified extract were determined by the Folin-Lowry assay (Lowry et al., 1951) using Bovine serum albumin (2 mg/ml)) as a standard. The absorbance at 540 nm was used to estimate protein content in aqueous, saturated fractions and purified extract.
was isolated from prawn body. Larvae were fed twice per day with probiotic mixed feed for treatment group. As compared to the control group, significant reduction of V. harveyi growth was observed at 8 hr and at 50 days in in-vitro and in-vivo challenge test, respectively. Treatment group showed better performance than the control one in terms of growth, digestibility and immunity. The weight gain at probiotic treated prawn at the end of the experiment was recorded as 549.30±40.02% (specific growth rate SGR 3.12±0.10), while it was 310.77±18.68% (SGR 2.35±0.08) in control group.
Those which aggregated with salt particles and formed clumps were considered hydrophobic. E.coli grown on MacConkey agar plates were inoculated into 1 ml of Phosphate buffer saline (PBS) at pH 6.8 and turbidity was matched with McFarland tubes 6 or 7 to get a colony count of 5x109 colonies/ ml. Different molar concentrations of ammonium sulphate namely 0.625M, 1.25M and 2.5M were prepared. One loopful (10µl) of bacterial suspension made in PBS was mixed with equal volume of ammonium sulphate solutions of different molarity on a clean glass slide and observed for one minute while rotating. The highest dilution of ammonium sulphate solution giving visible clumping of bacteria was noted as the salt aggregation test value.
Oral mucositis is the main complicate of head and neck chemotherapy. The current study was conducted to evaluation of Hypozalix artificial saliva and propolis mouthwash efficiency for the prevention of Chemotherapy induced-oral mucositis in Leukemia patients .This was a randomized controlled clinical trial conducted on 72 Chemotherapy induced-oral mucositis in Leukemia patients. The patients were divided into 3 equal treatment groups: Hypozalix artificial salivary, Propolis Mouthwash, and control groups. The method of artificial saliva Hypozalix usage according to the manufacturer order was as 4 or 5 puffs every 8 hours and every time on the left or right buccal mucosa, and the second group method usage was for 14 days, everyday 3 times, each
All the extrats showed most prominent zone of inhibition against fungal strains like Candida albicans and Aspergillus niger when compared with standard drug Nystatin. (Refer Table 10) Aqueous extracts formulation and ethanolic extracts formulation showed 24mm and 21mm inhibition on Escherichia coli, 22mm and 21 mm for Bacillus subtilis, 26 mm and
MATERIALS AND METHODS The Present study was carried out in the Department of Microbiology ,SRM Medical college Hospital and research centre from 2012 to .A total of 128 consecutive isolates of Enterococci were obtained from clinical specimens like urine,pus,blood and other body fluids in the diagnostic section of Central Microbiology Laboratory and processed as per standard recommendation procedures. Inclusion criteria: All the Enterococcal isolates from clinical samples such as urine,pus,blood,wound swab,catheter tip and other body fluids are included. Exclusion criteria: All commensal enterococcal isolates from anatomical sites like gastro-intestinal tract,female genital tract and oral cavity are excluded. PROCESSING