Sdole Test

BIOCHEMICAL CHARACTERISATION:(Shirling and Gottlieb, 1966)

Indole test:

The selected actinomycete cultures were inoculated in to the tryptone broth. After inoculation the indole production was detected by adding kovac’s reagent (dimethyl aminobenzaldehyde). Cherry- deep red colour ring formation indicates positive result where as the formation of yellow colour ring indicates negative result.

Methyl red test Voges proskauer test

The selected actinomycete cultures were inoculated in MR-VP broth and after 48 hrs of incubation, to the one set of tubes 5 drops of methyl red indicator was added. If the colour of methyl red turned to red in the inoculated broth, it indicates positive reaction if it turned to yellow indicates negative reaction.To the other sets

The selected streptomyces culture was inoculated into the broth. The tubes were incubated 4°C, 10°C, 20°C, 28°C, 37°C, 45°C, and 60°C for 8-10 days. The growth of the inoculated cultures was recorded after incubation.

Effect of NaCl (Tresner et al., 1968)

Yeast extract-malt extract agar was supplemented with Nacl at different concentrations 4%,6%,8%,10%(W/V) and autoclaved.After autoclaving, the medium wsa poured into sterilized petriplates.A loop full of spore suspensipn of each selected streptomyces species was streaked on the solidified agar plates divided into five sectors and incubated for 8-10 days. After the incubation period, the sodium chloride tolerance of the selected streptomyces was determined.

Utilization of Sugars as Carbon Source: (Shirling and Gottlieb, 1966)

The basal medium was prepared with carbon sources like glucose, fructose, arabinose,glactose, xylose, mannitol, sucrose and maltose in test tubes separately and autoclaved. The test tubes after autoclaving, were inoculated with the selected streptomyces species and incubated at 37 c for 8-10 days.The growth of the cultures in the test tubes were recorded after 8