Enterobacteria Case Study

In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates. Each plate serves as a first step to identify the unknowns. The TSA (tryptic soy agar) can be used to do a gram stain, which differentiates gram-negatives from gram-positives, based on the structural make up of the cell wall (Carson, 2015). The blood agar plate is used to test for hemolytic activity, which is useful for distinguishing gram-positives. A MacConkey plate is selective by inhibiting the growth of gram-positives and differential due to the fermentation of lactose by certain gram-negative species.

If there are 5 different types of bacteria how many strains could there be? 4. Why did the author title it the demon under the microscope? 5. Are there any infections the sulfa drug can’t cure? 6.

Sporadically, this can become serious and progress into pneumonia. A bacterial infection can be treated by a round of antibiotics but can occasionally become life-threatening, particularly in the frail and elderly. However, because some bacterial infections can produce signs and symptoms similar to influenza, bacterial infections should be considered and appropriately treated, if suspected.

Introduction Our world is composed of many bacteria’s’ that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods that have been learned in the laboratory to determine our unknown bacteria. Bacteria’s can have different features, shapes, and or arrangements that help microbiologist determined their role in our life (whether they are good or bad for humans).

In this case, chronic respiratory tract infections caused by Pseudomonas aeruginosa especially when the infection occurs in both either bronchiectasis and cystic fibrosis. These are one of the most difficult infections that are very hard to control. These serious complications requires intensive care hospitalisation or sometimes it can be fatal. This is because it is well known that patients with either infections, often develop acute exacerbations with viral or bacterial superinfection. Exacerbations means worsening of COPD symptoms such as shortness of breath, quantity and colour of phlegm that usually typically lasts for several days.

There has recently been an outbreak of a new disease in South America known as Ashella schmiddy. Recent studies that have been released have shown that A. schmiddy is a highly infectious flesh eating bacterium. A. schmiddy is a Gram Negative(-) bacillus, and it’s preferred portal of entry is the skin. It has been proven that once the bacteria is introduced to even the smallest cut or abrasion on the skin, infection quickly follows. One thing that makes this new disease so worry-some is the number of invading microbes that it takes to infect 50% of the population, which is only 15-45 cells, which is an extremely low dosage.

Q1A: What is the mechanism of action of colistin? Colistin is an antibiotic that works best against Gram-negative bacteria. It works by binding to LPSs (lipopolysaccrides) and phospholipids in the outer cell membrane of the bacteria. This, in turn, disrupts the outer cell membrane by displacing cations and leaking the intracellular contents, combining it with outer cellular contents, causing the bacteria to be unable to differentiate the bacteria’s intra and outer cellular contents from one another.

Asthma is the swelling that causes you to wheeze making you feel as you can’t breathe it can be treated by epinephrine. Pneumonia is an infection of the lungs caused by bacteria and sometimes it can be treated without treatment. Bronchitis is an infection in the mucus membrane, wheezing and chest pains are some symptoms and it can be treated by prescribing brunch dilator or antibiotics. Sinusitis pressure in the sinuses also nasal discharge treatments are antibiotics and many others. Tonsillitis symptoms include fever, hard for you to swallow and sore throats.

Clostridium difficile, also referred to as C. diff, is a bacterium that is known to cause mild to severe diarrhea and can lead to severe inflammation of the colon (Government of Canada, 2014). C. diff contains spores that are found in fecal matter. Through encountering a surface containing contaminants such as feces, puts a person at risk for the later development of diseases such as c. diff. This bacterium can also be spread through contact from healthcare workers that have not followed correct cleansing routines such as but not limited to hand washing. High dosages or extended usage of antibiotics can also increase the chances of developing C. diff (Government of Canada, 2014). Antibiotics change the number of bacteria found in the body, in this case the gut, and in result to the lowered number the bacterium has a higher chance of survival and production of

By Gram staining alone, it was safe to eliminate the three Gram positive bacteria that could have been assigned: S. epidermidis, M. luteus, and B. megaterium. The second step was to streak plate Unknown #10 to observe its macroscopic

The healthcare-acquired infections get much attention from the Joint Commission due to their frequent occurrence (Chinn, 2014). The focus is given to hand hygiene and protocols to address specific infections (Chinn, 2014). The infections cited include reducing central line bloodstream infections, surgical site infections, multi-drug resistant organisms, and catheter-associated urinary tract infections (Chinn, 2014). In the article’s discussion

Although the nature of this kind experiment was unable to provide us with numerical data, our graph was constructed using the average percent of E. Coli killed for each pair of cultures. To determine these percents for each substance we used the process of elimination. We put the two petri dishes side by side and used a checklist ranging from 1-100% to narrow down the possibilities. After obtaining a small range of possible percents, we made an estimate to determine the percent. After doing this for both the of cultures for that pair we averaged the two numbers. We repeated this for all of the culture pairs in our experiment.

INTRODUCTION: Quick look at your hands do you see them. (attention) Do I see what you might be asking? Well the millions of bacteria that are currently hanging out on your hands.

S. aureus is a non-motile facultative anaerobic bacteria. This bacteria can survive long periods in the environment; so it is readily transmissible. S. aureus causes a number of medical infections and is the leading cause of wound infections. It is an opportunistic bacteria, and infects any population. It favors the skin, since it is part of the normal flora.

In all areas of healthcare, particularly in acute care settings, patients may be unable to care for their nutritional and/or hygienic needs properly. One area that is especially important to address is the perineal care of patients who are incapacitated, or otherwise unable to care for their hygienic needs independently. Normal bacterial flora can develop into an opportunistic infection if the bacteria reaches certain areas of the body. For example, a common cause of urinary tract infections (UTI) is due to Escherichia coli from the colon coming into contact with the urethra (Copstead & Banasik, 2013). If the patient is immunocompromised, elderly, sedentary, or otherwise compromised in their abilities to void, this can predispose the patient