Once a prevalent color change had been observed at approximately 4 minutes (blue green color) the crucible was set on the counter using the tongs to cool for approximately 5 minutes. The appearance after this period resulted in another color change back to white. The crucible, lid, and hydrated copper sulfate was weighed again to calculate the mass of water lost by dehydration (described in table 1.3). This was done by subtracting the final mass by the initial mass of the crucible, lid, and compound. The mass of the crucible would remain unchanged while the mass of the compound would be altered.
I let the alcohol flow on 45-degree angle slide within 15 seconds and wash it with water to remove colors on the surface. Lastly, the unknown is once again dyed with safranin for 1 minute then wash it off with water for the last time and dry it using bibulous paper. After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster.
Compare the result to the chart on the back of the urinary pH test strips bottle, and record data. Clean the stirring rod with water before moving on to the next test tube. Repeat this process for each increment (2 mL, 3mL, 4mL) Figure #1: Picture of bean solution mixed Figure #2: Picture of materials needed for the with alpha galactosidase experiment Safety considerations: Be careful with the beakers, glass stirring rod, and test tubes, as they could break easily and can cause cuts in the skin. DCP: A scatter plot will be used to display how the amount of alpha galactosidase (measured in mL) in the bean solution affects the glucose concentration (measured in mg/dL) and error bars to show the standard deviation. A line of best fit will be used to show the relationship between the glucose concentration and the amount of alpha galactosidase.
The solution was discarded into the waste bin, and the materials were washed. The second reaction in Part B, sodium hydroxide and ammonium chloride, began by saving the data from the first reaction and setting up the LabQuest to new data collection under the same conditions as the first reaction. The cups were restacked and placed in the beaker. Using a graduated cylinder, 50mL 2M NaOH was added to the cup. The cup was then covered and the temperature probe inserted.
Digestion is a form of catabolism process of breaking down food physically and chemically large food molecules in to smaller components. Chemically digestion is carried out using enzymes and hormones with in different segments of the digestive tract. The presence of enzymes in the digestive tract helps breakdown polymeric biomolecules into individual monomers. This process is crucial for surviving because cells cannot use nutrients the way they were consumed without being metabolized. Nutrients need to be small enough to be absorbed by epithelium of the small intestine and transported by the help of carrier proteins.
Kidney failure is a disease when the organ function similar to dark red colored peas this decline. Dear Dr. Dr. SpPD, Endocrine Metabolic Division staff, Department of Pathology In Cipto Mangunkusomo Hospital, revealed there are some kidney function. First, as the synthesis of hormones, that regulates blood pressure and stimulation of the production of erythrocytes (eritropoitin). Second, set up bases balance through spending a acidic or alkaline urine. Third, balance water and mineral intake and or control the secretion of water and minerals such as sodium, calcium, potassium, and chloride.
Biuret test is a test which is utilized to indicate unhydrolyzed proteins. When there are peptides in a solution, a copper (II) ion forms violet-coloured coordination complexes in an alkaline solution. The biuret test can be utilised to analyse the concentration of proteins due to peptide bonds that occur with the same frequency per amino acid inside the peptide. In this experiment, the colour changed to purple to indicate the presence of protein. The pH was found to be 7, which is in the range of a healthy person’s pH (which is 7.4).Benedict`s solution is made up of alkaline copper sulphate and sodium citrate (blue in colour) (Danson and et al, 1996).
Experimental Methods: 1. SYNTHESIS OF 4-BENZOYL BUTYRIC ACID METHYL ESTER Materials required * 5 oxopentanoic acid : 2 gm (Aldrich) * Methanol : 50 ml * Acetic Acid (Rankem) Procedure: * 2 grams of 5 oxopentanoic acid was weighed and placed in a round bottom flask and then to it 50 ml of methanol was added. It was placed on a hot plate and the temperature was increased to 50 degrees under ambient air conditions. * To the RB, 2 ml of acetic acid was added and then by attaching a condenser the entire reaction was put on refluxing at 70 degrees Celsius in an oil bath. * For work up: * The reaction media was concentrated till about 10 ml and then dry silica gel was added.
We started of by adding a small piece of liver to an empty test tube and placing just enough water to cover it. After this we placed it in a hot bath for five minutes and after letting it cool we tested to see its reaction rate with hydrogen peroxide, it received a one because of the very little reaction occurring. Then we got four more empty test tubes and filled two of them with hydrogen peroxide and two of them with equal amounts of liver. Then we placed a test tube with hydrogen and another test tube with liver into an ice bath and placed the remaining into a warm bath for three minutes. After this we tested the reaction rates by pouring the respective hydrogen peroxide and live (warm with warm and cold with cold).
FTIR Spectroscopic Study on Quantitation of Urea in Human BloodSerum Abstract: The quantitation of urea has been achieved using FTIR spectroscopy. The FTIR spectra of human blood serum samples are recorded in Mid IR region 4000-400 cm-1.The normal blood serum is treated with urea at different concentrations and FTIR spectra are recorded, which confirm the specific peaks related to urea. A plot between concentration of urea and percentage of absorbancehas shown linear relationship. The study being complementary to chemical analysis is very much useful for the estimation of urea in the blood serum of patients suffering from diabetes and renal diseases. Key Words: FTIR Spectroscopy, Quantitation, Human blood, Serum, Urea.