Sex Hormone Lab Report

Introduction Gender is the state of being a male or a female. The differences between the two categories are known to lie within the reproductive system and certain physical attributes. However, these differences are not limited to genitalia and physical features. The presence of testosterone (male sex hormone) or estrogen (female sex hormone) affects metabolism, behavior, the functioning of organ systems, and the development of various diseases. For example, males tend to have higher metabolic rates than females. It is also known that females have a lower risk of developing obesity related metabolic disorders than males. Further research pertaining to this topic can allow people to understand the effects of sex hormones on functions other

To start the lab, all mice should be weighed using the beaker and the balance. The weights should be recorded, as they will be incorporated into the results later. Take one of the male mice, place it in the chamber, seal it tightly with the chamber cap, and place the chamber into the bath. Run the leak test. If the test falls between -.300ml and +0.300, you can proceed to the next step. Set the Micro-Oxymax software to record data every minute for 20 minutes. Create a scatterplot on excel and create an equation using the trend line. This will represent the metabolic rate. Take the mouse out of the chamber and repeat this process with the 4 other male mice and the 5 female mice. Then, inject the 5 male mice with 0.5 mg of estrogen and inject the 5 female mice with 0.5 mg of testosterone. Put all the mice back into their tanks for the night. The next day, repeat the steps involving the chamber and Micro-Oxymax software. Take the data from before the hormone injections for the male mice and compare them to the data collected after the injections. Find the t-value and p-value and test the hypothesis with a significance level of 0.05. Do the same for the female mice and reject or accept the null