Siderophore Lab Report

1502 Words7 Pages

Transport of siderophores inside the cell. Siderophores form complex with free iron and carry it inside the cell with the help of membrane receptor molecules. In fungi four mechanisms have been proposed for transport of siderophore bound iron to the cells.
1. Shuttle mechanism in which the Fe (III) siderophore complex is transported across the cell membrane by siderophore specific transport proteins and the iron is reductively removed from the siderophore complex inside the cell (Helm & Winkelmann 1994).
2. Taxicab mechanism Fe (III) is transferred from extracellular siderophores to intracellular ligands across the cell membrane (Winkelmann and Huschka 1987).
3. Hydrolytic mechanism- Fe (III) siderophore is transferred inside the cell by …show more content…

Tetraglycylferrichrome is a cyclic heptapeptide made up of three ornithine and four glycine residues; the acyl group is acetyl (Deml et al . 1984). For all other members of the ferrichrome family the general formula is -Orn1-Orn2-Orn3-A-B-Gly-, where Orn1–3 areNd -acyl-Nd -hydroxy-L -ornithine units and A and B are residues of alanine, serine or glycine (Hossain, Jalal & van der Helm 1997). The ornithine residues are linked into a cyclic peptide by three amino acid residues, forming a macrocylic ligand (Jalal & van der Helm 1991, van der Helm &Winkelmann 1994). The acyl side chains on the ornithine residues are homogenous except in asperchromes B1 to F3; this is a group of minor siderophores only produced by Aspergillus ochraceous (Jalal et al . 1984, Winkelmann 1990). These siderophores all have -[Orn]3 -[Ser]2 -Gly- as the peptide backbone (Winkelmann & Huschka 1987, Jalal et al . 1988a) and combinations of acetyl and trans –anhydromevalonic acid residues as the acyl groups (Winkelmann &Huschka 1987, Leong & Winkelmann 1998). Ferrichrome itself has three glycine residues in the backbone (Emery &Neilands 1961). All of the other ferrichromes contain different combinations of serine and glycine (Deml et al . 1984, Jalal et al . 1984), except for malonichrome and ferrichrome C which contain

More about Siderophore Lab Report

Open Document