The solvents DMF and methanol were distilled for purification. Other chemicals were used as obtained. 2.2 Preparation of polystyrene (PS) Polystyrene prepared by free radical polymerization of styrene monomer. Styrene (1 mole) was taken in a round bottom flask (RBF) fitted with a reflux condenser. DMF was used as a solvent and AIBN (0.5% w/w of total monomer) as free radical initiator .The reaction was carried out at 70±2° C for 6 hour with constant stirring.
Exercise 1 1. Suppose a household product label says it contains sodium hydrogen carbonate (sodium bicarbonate). Using your results from Data Table 1 as a guide, how would you test this material for the presence of sodium bicarbonate? B BoldI ItalicsU Underline Bulleted list Numbered list Superscript Subscript33 Words If I had a household product labeled sodium bicarbonate, I would add an acidic substance and expect bubble to be created. As we know acid reacts with bubbles when combined with sodium bicarbonate.
The samples were filtered by vacuum over pre-weighed and wetted glass fiber filters (24 mm GF/C; Whatman; Kent, UK). The samples on the filters were rinsed with 5 ml stop solution to remove non-specific, cell surface binding of 3H-FLC. The filters with fungal balls were either allowed to dry for 24-48 hr or were baked in a drying oven for 15 minutes at 95° C. Each dried filter containing fungal balls was then re-weighed to obtain the dry mass of each fungal sample. The filters were finally transferred to 5 mL scintillation vials containing 3 ml of scintillation cocktail (Ecoscint XR, National Diagnostics, Atlanta GA). Radioactivity associated with the fungal sample on each filter was measured in a liquid scintillation analyzer (Beckman Coulter, LS 6500 multipurpose scintillation
Isolation of Ecdysterone from Sesuvium portulacastrum As detailed in Figure1, Ecdysterone was isolated from Sesuvium portulacastrum using a sequential extraction process with Chloroform (CHCl3) followed by Methanol (MeOH), after which Alumina column chromatography of MeOH extract was performed. The different fractions were then eluted using varying proportions of CHCl3 and MeOH, resulting in MeOH: CHCl3 (20:80) bioactive fraction. Thin-layer chromatography (TLC) analysis of MeOH: CHCl3 (20:80) fraction was carried out using a solvent system containing MeOH (15%) and Ethyl Acetate (85%). The TLC plates were visualized by spraying with vanillin- HCl reagent resulting in a UV sensitive band, which on heating, developed green color. The UV sensitive bands were purified using repetitive preparative TLC followed by crystallization.
((Love J). sodium picosulphate in combination with magnesium citrate has synergistic effects. As mentioned earlier, sodium picosulphate has purgative actions, while magnesium citrate (osmotic laxative) reacts with water thereby retaining fluids in the colon. By these dual mechanisms sodiumpicoslphate/magnesium citrate exerts its bowel cleansing effects. Various clinical studies evaluated its efficacy in comparison with other bowel preparation agents: • In a randomized, multicentre, assessor-blinded, prespecified non-inferiority, head to head study, bowel cleansing effects of sodium picosulphate/magnesium citrate were compared with that of 2L polyethylene glycol solution and two 5-mg bisacodyl tablets.
(2006), after slight modifications. The fundamental principle of the DPPH method is the reduction of the DPPH radical in an ethanolic solution by an H-donator antioxidant (AH) to form the non-radical form DPPH-H. In a microtube, 10 µL of each fraction at different concentrations (10 - 1000 µg/mL) were mixed with 990 µL of a DPPH solution (0.1mM) prepared daily. The reaction was allowed to develop for 30 minutes in the dark at room temperature, and then the absorbance was read at 515 nm with a spectrophotometer (Spectronic Helios Alpha UV-Visible, Thermo Electron Corporation, U.S.A). The analysis was done in triplicate for each
Straight afterwards, saline solution was added (NaCl 0.16 M, pH=7) to the 10ml of the yolk solution with a Pasteur pipette, avoiding the sample diffusion, forming two phases and filling the tube completely. The ultracentrifugation was carried out during 19.5 h at 4ºC and 45000 rpm. (244.500 x g) in a Kontron Centrikon T-2190 ultracentrifuge in a TFT 50.38 rotor,
Abstract In this experiment, the reaction kinetics of the hydrolysis of t-butyl chloride, (CH3)3CCl, was studied. The experiment was to determine the rate constant of the reaction, as well as the effects of solvent composition on the rate of reaction. A 50/50 V/V isopropanol/water solvent mixture was prepared and 1cm3 of (CH3)3CCl was added. At specific instances, aliquots of the reaction mixture were withdrawn and quenched with acetone. In addition, phenolphthalein was added as an indicator.
5.2.3 Incorporation of Emulsion into Gel base For preparation of emulgel, the obtained emulsion was mixed with gel in 1:1 ratio with gentle stirring. During mixing of emulgel, glutaraaldehyde was mixed to obtain the emulgel (Figure 5.1). Fig 5.1: Flow chart for preparation of Emulgel 5.3 OPTIMIZATION OF AZATHIOPRINE LOADED EMULGEL Various process variables like gelling agent concentration, oil and emulsifier concentration which could affect the preparation and properties of emulgel were identified and studied. 5.3.1 Effect of varying Gelling Agent concentration Emulgel was prepared using varying concentration of gelling agent via 0.5, 1.0, 1.5 and 2.0 gm while keeping other variables constant by method reported in section. The effect of varying gelling agent concentration on viscosity and drug content are reported in Table 5.1 and shown in Figure 5.2 and 5.3 respectively.
Ltd. 4 Melting point Sentwin India 5 NMR Bruker Advance II 400MHz 7 Heating Mantle Inco 6 Structure builder Chem draw Ultra 8.0 4.2 Experimental work: 4.2.1 General procedure for Chalcones: 2’-hydroxy acetophenone or 2’-hydroxy propiophenone (0.2ml) and substituted benzaldehydes (0.5 g) were mixed in the round bottom flask. After that 40% NaOH solution (4g NaOH in 10 ml of distilled water) and ethanol were added in round bottom flask. The reaction mixture was stirred upto 6-48 hours. Completion of reaction was monitored in TLC plate (n-Hexane: Ethyl acetate 9:1). The reaction mixture was poured into ice cold water acidify with 1% HCl and precipitates were collected, filtered and dried and recrystalized with ethanol.