Specific Activity Research Paper
4.3) Briefly explain what you understand by specific activity of an enzyme and how you could measure it. (P 4.3)
Specific activity is a term utilize to measure the rate of reaction of an enzyme with a substrate. Specific enzyme activity is a measure of enzymes purity and quoted as units/ mg. the value becomes huge as an enzyme preparation becomes purer since the amount of protein (mg) is typically less, but the rate of reaction stays the same/ may increase due to reduced interference/ removal of inhibitors.
SPECIFIC ACTIVITY MEASURING ENZYME KINETICS
Enzymes are proteins that act as catalysts in reducing the amount of activation energy for a reaction to take place, activation energy that is needed for get reactions begins since several reactions. Do not take place at all. If they thermodyanamically possible. Enzymes catalyse specific reaction in a particular site known as the active site. Where the reactants binds and react to generate the product. Substrates have active
…show more content…
Specific activity is an essential measure of enzyme purity. Various batches of a pure enzyme must have the same values and even diluting an enzyme solution several times will have identical specific activity values even though there will be various enzyme activity values. This is because in calculating specific activity, the numerator (units/ ml) and denominator (mg/ ml) are affected equally.
Specific activity is very difficult from activity but the calculation of specific activity is still dependent on the activity value. This means that the stated specific activity activity value. This means that the stated specific activity value will also be dependent on the enzyme unit definition. Batches of enzymes that have lower than expected specific activity value may contain enzyme molecule that have become altered or mixed with impurities. ( Lecture
Ap Biology Review Sheet
855 Words | 4 PagesCofactor- Molecules that aren’t proteins nor organic, but help make the reaction go faster when they connect to the active site. 9. competitive inhibitor- prohibits the reaction from taking place by going into the enzyme’s active site so the substrate can’t. 10.
Horseradish Peroxidase Lab Report
1090 Words | 5 PagesThe products are released from the enzyme surface to regenerate the enzyme for another reaction cycle. The active site has a unique geometric shape that is complementary to the shape of a substrate molecule, similar to the fit of puzzle pieces.
Beta Galactosidase Lab Report
494 Words | 2 PagesEnzymes are proteins that significantly speed up the rate of chemical reactions that take place within cells. Some enzymes help to break large molecules into smaller pieces that are more easily absorbed by the body. Other enzymes help bind two molecules together to produce a new molecule. Enzymes are selective catalysts, meaning that each enzyme only speeds up a specific reaction. The molecules that an enzyme works with are called substrates.
Enzyme Concentration Lab Report
1515 Words | 7 PagesUnder some conditions, substrate will bind to the active site of an enzyme and form an enzyme-substrate complex. The enzyme would fasten the chemical reaction and the substrate will
Enzyme Turnip Peroxidase Lab Report
1117 Words | 5 PagesAll enzymes are under the class of protein biomolecule. Amino acids are the basic units that are combined to make up an enzyme. The biomolecule that stores information is a Nucleic Acid. The specific 3-D region within an enzyme is called the active site. The chemical
Substrate Concentration Lab Report
181 Words | 1 PagesHypothesis: Increasing substrate concentration will increase the initial reaction rate until it stops increasing and flattens out. Independent Variable: Substrate concentration Dependent Variable: The substrate itself, 1.0% Hydrogen Peroxide How Dependent Variable will be Measured: Hydrogen Peroxide will be used in every experiment, just with different test tubes. The amount of Hydrogen Peroxide in the mixing table is the amount that will be added to each test tube.
Enzyme Fast Lab
485 Words | 2 Pages3. Look at your graph for Part B, how does temperature affect enzyme activity? The colder the temperature the greater the reaction. 4. Look at your graph for Part C, how does pH affect the enzyme activity?
Lab Report On Enzyme Activity
1419 Words | 6 PagesLABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.
Laboratory Report on Enzyme Activity
1407 Words | 6 PagesLABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
B-Galactosidase Lab Report
1764 Words | 8 PagesBy using a spectrophotometer to measure absorbance at 420 nm, the rate of enzyme activity after all reactions have come to a stop can be
The Biological Temperature Of Catalase
750 Words | 3 PagesIntroduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).
Effect Of Temperature On Amylase
1167 Words | 5 PagesThese enzymes have a secondary and tertiary structure and this could be affected by increases and decreases in temperature beyond the optimum temperature of the enzyme to work in. Mostly enzymes are highly affected any changes in temperature beyond the enzymes optimum. There are too
Alkaline Phosphatase Enzyme Lab Report
2575 Words | 11 PagesIntroduction 1.1 Aim: To determine the kinetic parameters, Vmax and Km, of the alkaline phosphatase enzyme through the determination of the optimum pH and temperature. 1.2 Theory and Principles (General Background): Enzymes are highly specific protein catalysts that are utilised in chemical reactions in biological systems.1 Enzymes, being catalysts, decrease the activation energy required to convert substrates to products. They do this by attaching to the substrate to form an intermediate; the substrate binds to the active site of the enzyme. Then, another or the same enzyme reacts with the intermediate to form the final product.2 The rate of enzyme-catalysed reactions is influenced by different environmental conditions, such as: concentration
Exploring Factors That Affect The Rate Of Enzyme Activity
1043 Words | 5 PagesEnzymes are catalysts that accelerate chemical reactions by decreasing the amount of activation energy needed, during reactions enzymes are not consumed allowing the enzyme to be reused (Eed, 2013). Each individual enzyme has a groove on its own surface, this groove is known as the active site (Robinson, 2016). On the active site a reactant, also known as a substrate, interacts with the enzyme in order to cause a reaction that could take days or years to happen occur significantly faster (Robinson, 2016). There are many factors that affect how efficient enzymes are, such as pH, temperature, and both enzyme and substrate concentration (Eed, 2013). Another factor is charge, however its affect tends to be negligible and the actual shape of the
Effect Of Enzyme Concentration On Enzyme Reaction
1046 Words | 5 PagesABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.
More about Specific Activity Research Paper
Ap Biology Review Sheet
855 Words | 4 PagesHorseradish Peroxidase Lab Report
1090 Words | 5 PagesBeta Galactosidase Lab Report
494 Words | 2 PagesEnzyme Concentration Lab Report
1515 Words | 7 PagesEnzyme Turnip Peroxidase Lab Report
1117 Words | 5 PagesSubstrate Concentration Lab Report
181 Words | 1 PagesEnzyme Fast Lab
485 Words | 2 PagesLab Report On Enzyme Activity
1419 Words | 6 PagesLaboratory Report on Enzyme Activity
1407 Words | 6 PagesB-Galactosidase Lab Report
1764 Words | 8 PagesThe Biological Temperature Of Catalase
750 Words | 3 PagesEffect Of Temperature On Amylase
1167 Words | 5 PagesAlkaline Phosphatase Enzyme Lab Report
2575 Words | 11 PagesExploring Factors That Affect The Rate Of Enzyme Activity
1043 Words | 5 PagesEffect Of Enzyme Concentration On Enzyme Reaction
1046 Words | 5 Pages