I let the alcohol flow on 45-degree angle slide within 15 seconds and wash it with water to remove colors on the surface. Lastly, the unknown is once again dyed with safranin for 1 minute then wash it off with water for the last time and dry it using bibulous paper. After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster.
* In the above RB, a calculated amount of 1.2 equivalent amount of PTSH was added during continous stirring. * To the RB a condenser was attached and it was put on refluxing for a time period of 18 hours at a temperature of 80 degrees in an oil bath. * Post refluxing, the condenser was removed and it was left undisturbed for 1 day and then cooled to a temperature of -20 degrees by keeping in the freezer for setting of hydrazone crystals. * Once the crystals were formed, the supernatant was decanted and the crystals were transferred to a beaker where they were washed with cold methanol and then dried. * These dried hydrazone crystals were used in the subsequent steps.
5 mL of 3M sodium hydroxide, 5 mL of de-ionized water, and 15 mL of hexane were added to the reaction flask and stirred. The mixture was transferred to a separatory funnel, separated into an organic layer and water layer, and then drained. The water layer was washed twice with 10 mL of hexane. The organic layer was dried
Once 3-4 readings for the solution were collected, the 2M NaOH was added to the solution. The lid was quickly replaced in order to prevent heat from escaping and not being recorded by the temperature probe. The cup was swirled until the temperature reached a peak and began decreasing. After the 180s had passed, data collection ended. The solution was discarded into the waste bin, and the materials were washed.
This was repeated until no more gas was released. Next, the funnel was suspended through a ring, and 10 ml of 5% sodium hydroxide was added. When the two layers were separated in the separatory funnel, the aqueous layer was identified. The two layers were then separated into two different beakers. The water layer was acidified by adding concentrated hydrochloric
N-(1-Carboxymethyl-1H-tetrazol-5-yl)-hydrazinium nitrate (3). A solution of AgNO3 (0.10 g, 0.60 mmol) in distilled water (1.5 mL) was added dropwise in the dark to the solution of compound 2 (0.10 g, 0.60 mmol) in Deionized water (1.5 mL) under stirring. After 2-3 hour, the precipitate was filtered, and rinsed with 4 mL distilled water. The solvent was removed by rotary Evaporation to produce a white solid at 88% yield (0.10 g); N-(1-Carboxymethyl-1H-tetrazol-5-yl)-hydrazinium nitrate: Yield: 88%; yellow crystals;. IR (KBr): 3396, 3329, 3140, 3008, 1628, 1494, 1383 cm-1; UV (H2O): λmax = 293-296
The chloride ion is more polar since it is above bromine on the periodic table and is more prone to hydrogen bonding due to its smaller size. Chloride ions are worse than bromine ions for nucleophilic attack, because the chloride ions are fully solvated and are not as available to attack. This is why Bromine ion is better nucleophile because is less electronegative and is willing to give up electrons. 3. What is the principal organic by-product of these two reactions?
Water was removed from the dish, and the Cu was then washed thrice with 5 mL deionized water, and decanted between washings. It was then similarly washed thrice with 5 mL of ethanol. The evaporating dish was then placed on the steam bath to dry until the Cu had a sand-like texture. The bottom of the dish was dried, it was cooled to room temperature, and then its mass was measured and recorded. All the solutions were disposed of in the liquid waste container, the Cu and Al were disposed of in the solid waste container, and the apparatus was cleaned and put
Once AMD reached the coveted pH level, it was filtered using filter paper (0.45 μm) to obtain the precipitate. The filtrates were then measured for the EC level using conductivity meter, TDS level using TDS meter, and concentration of Cu2+ using PerkinElmer Atomic Absorption Spectroscopy (AAS) Analyst 400. All analyses were conducted in Analytical Chemistry Laboratory, University of Mataram. Filtrates (with several pH levels) found to still contain Cu2+, would be treated to the sulfidization treatment. Sulfidization treatment using SNW from Sebau This experiment was conducted by adding pure SNW from three sampling points (T1, T2, and T3) to the AMD with three different pH levels in 1:1 ratio reaction.
Shake funnel and rinse off water layer ( This contains the sulfuric acid and majority of methanol). Again wash the ether with 25ml of water and then wash the organic layer with 25ml of 10% sodium bicarbonate to extract unreacted benzoic acid. Again shake separatory funnel with frequent venting of pressure and opening the stopcock. Allow the separation of layers and drain off bicarbonate layer into a beaker. Wash ether layer with saturated sodium chloride solution and retain ether layer.
The silver ion TLC was prepared through the following procedure: Silver nitrate was dissolved in 10 ml of distilled water. This aqueous solution of silver nitrate was absolutely mixed with 9 g of silica gel (10 ~ 40 μm particles). Then, a 10 × 5 cm TLC plate was coated with the above slurry and activated for 1 h at 90 °C before use. They were immediately transferred into a desiccator in dark for storage after cooling. 32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate.
Two chemical reactions are carried by adding sodium hydroxide to the acidic solution from Part I. During the first reaction is the neutralization of the excess of nitric acid in the mixture by sodium hydroxide. The second reaction takes the place after naturalization is a complete and NaOH is in excess. While the liquid inside the beaker is being stirred, with the stirring rod, 10 ml of 6 M NaOH is poured into the solution from Part I at 1 mL at a time. After each 1 mL the solution is tested for acidity with red litmus paper.
Purpose The purpose of this experiment was to evaluate the stoichiometric relationship between the testing agents and to identify the products formed. The relationship was found by completing three acid and base neutralization reactions using phosphoric acid, which is a triprotic acid, with different volumes of sodium hydroxide. Introduction Procedure Phosphoric acid solution with a volume of 1.00 mL and a molarity of 6.00 M was transferred into a 125-mL Erlenmeyer flask using a volumetric pipette. Sodium hydroxide solution with a volume of 6.00 mL and a molarity of 3.00 M was transferred into a 50 mL beaker using a volumetric pipette. While swirling the phosphoric acid solution in the Erlenmeyer flask, the sodium hydroxide solution was added to it a few drops at a time using a disposable plastic pipette.
Next, transfer the sample to a 50 mL beaker and mixes with distilled water, which gets by rinsing the crucible with its cover in 8mL, so the solution is generated. After that, put an aluminum wire into the beaker, and after a certain period of time the solution gains color. To finish the reaction, 5 drops of 6M of Hydrochloric acid is added into the beaker to clean the solution, which means that acid dissolves all salts of aluminum that is on the solution. After finishing the chemical process, collect and use the Butcher funnel to wash the cooper because it is going to be used to a vacuum filtration. After finishing the filtration, measure the weight of the sample and dry it.