The exploration was meant to also analyze the effect that temperature has on the rate of a chemical reaction. From the raw data obtained, it is clear that the time taken to complete the reaction decreases with increase in temperature. The effect of temperature on the rate of reaction is however dependent on the activation energy. When the activation energy is positive, as the temperature will increase , the rate of reaction will also increasing meaning that they are directly proportional .However, if the activation energy is negative, the rate of the chemical reaction will decrease as the temperature is increased. To carefully describe the relationship between the rate of reaction and the temperature, a graph of these two variables is plotted.
The substrates will be reduced guaiacol and hydrogen peroxide (H2O2). This reaction will produce oxidized guaiacol, oxygen gas, and water. In the oxidation of guaiacol it will change colors, this is what shows the reaction has occurred. To monitor the reaction, a spectrophotometer will be used that will measure the absorbance of light. If the substance is a darker color, it will absorb more light, and if it is lighter it will absorb less light.
Basically , enzyme speeds up the speed of a reaction by cause of at least a million as in comparison to the rate of the same reaction without the enzyme. Enzymes are extremely specific. Typically a particular enzyme catalyzes only a single chemical reaction or a set of closely related chemical reactions .For any catalyst enzymes do not change the equilibrium point of the reaction. The enzyme alters the forward and reverse reaction by specifically the same factor. One reason for the efficiency and specificity of an enzyme is the way the enzyme interacts with reactant molecule also known as the substrate.
Enzymes are globular proteins folded into a complex 3-dimensional shape that contain a special surface region called the active site where specific substrate can bind structurally and chemically. They act as catalysts, meaning that they are substances which lower the activation energy required for a chemical reaction to occur and therefore increases the rate of the reaction. Activation Energy is the minimum energy barrier needed to be overcome before a reaction can occur by providing an alternative reaction pathway. The beneficial aspect of enzymes is that they are extremely efficient and may be used repeatedly. One enzyme may be used to catalyze thousands of reactions every second.
Factors affecting the rate of Enzymic Reactions Enzyme concentration:- The rate of an enzymatically-controlled reaction is directly proportional to the enzyme concentration under constant conditions if an excess of free substrate molecule is present. As the concentration of the enzyme increases, the rate of reaction will also increase.Thus, enzyme –substrate interaction follow the mass-action law. Substrate concentration:- The rate of reaction is slow at very low concentration of substrate, but increases with the increase in substrate concentration up to certain concentration, where there is no increase in enzyme activity with the increase in substrate concentration. Eventually, a maximum is reached, and further addition of substrate has
Add 1 ml of diluted amylase extract to the reaction mixture. Starting with well number 0, add one drop of amylase reaction mixture to a different well each minute until the achromic point is reached (no color change). Repeat the experiment using boiled control extract instead of DAE for the determination of amylase
Low temperatures, can cause enzymes to slow down and decrease their rate of interaction with substrates. The structure of an enzyme are chains of amino acids, and have a specific shape that allow chemicals to react with the enzyme. Enzymes are natural atoms that altogether speed up the rate of essentially all of the chemical reactions that take place inside cells. A lab was conducted to test the effects of different diets on enzyme
In extreme conditions, the liquid inside the cell can solidify, forming crystals that pierce the membrane and kill the cell (“The Effect of Temperature on Cell Membranes,” Sciencing.). Temperature can also affect vital parts of the cells, such as enzymes. A small increase in temperature can increase enzyme activity, but too large of a change can denature the proteins, causing them to lose their function. Likewise, lowering the temperature too far can cause enzymes to slow their function until it is near nonexistent (“Temperature, pH and Enzymes,” GCSE
During an Enzyme substrate reaction, the speed of the Enzyme increases while increasing the concentration of the substrate. Increasing Enzyme concentration will increase the rate of reaction as more Enzymes will be colliding with substrate molecules, so more product will be