This reaction uses the enzyme aconitase. This is a reversible reaction. Iso- citrate is formed through the intermediate formation of cis-aconitate. Aconitase is an iron sulphur enzyme. In step three iso-citrate is oxidised to α-ketoglutarate and also CO2 is released.
The Bile Esculin agar test has its medium as selective and differential. Black medium shows a positive result for esculin hydrolysis. In the agar, Gram-positive cannot grow in the presence of bile while certain Gram-negative bacteria can hydrolyze esculin with bile present. MR-VP broth contains glucose and peptone. The enteric bacteria will oxidize glucose for ATP, but there are different fermentative pathways that allow glucose to be fermented.
Controlled Variables temperature, pH, sucrase + sucrose incubation time 4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. I believe glucose and fructose was used as an indicator because they are what produces sucrose and sucrose creates more sucrase activity. 5. Explain why denatured sucrase was used as a control.
EC 3 are hydrolases, which forms two products from the substrate via hydrolysis. (Bach, et al. 1961) This is seen in the equation: L- Arginine + H2OL-Ornithine + Urea (Nelson and Cox 2008). The urea cycle is the procedure where ammonia is transformed into to urea. Throughout the urea cycle, the amino acid, arginine, is changes into ornithine- this is another amino acid when hydrated, that is when water was added.
A line of best fit will be used to show the relationship between the glucose concentration and the amount of alpha galactosidase. Table 2: Glucose concentration based on the amount of alpha galactosidase in bean solution Amount of a-galactosidase (mL) Glucose concentration (100mg/dL) Average glucose concentration Standard Deviation Trial 1 Trial 2 Trial 3 Trial 4 Trial
(Cite %). DMT2 is a result of the body’s improper use of Insulin. Foods containing carbohydrates produce sugar that the body process’ for energy. The sugar is called glucose and is absorbed into the blood stream once the food is digested. Cells in the body absorb the glucose to provide the needed energy.
The glucose in your blood comes from carbohydrates in your food. Carbohydrates include sugar and starchy foods like: bread, pasta and rice. Keywords: Carbon dioxide, Glucose, Water, Oxygen and energy. Word and Symbol equations: Glucose + oxygen = Carbon dioxide + water C2H12O6 + 602 = 6CO2 + energy (ATP) Task 2 – Investigating Respiration You identify Carbon dioxide gas by putting a lighted wooden splint in a test tube of carbon dioxide and carbon dioxide turns limewater cloudy white.
Other disaccharides are broken down by other carbohydrase enzymes. Carbohydrates (starch)are broken down in the oral cavity by saliva amylose. They are made up of 3 elements, carbon (C), hydrogen(H) and oxygen(O) and can be found together in three different forms. Either as a monosaccharide, disaccharide or a polysaccharide they are differentiated by the number of rings in their chemical compound. There chemical formation is C6,H12,O6 and due to their bond angles between the carbons, tend to form a pentose of hexoses, stable ring structure.
In addition, phenolphthalein was added as an indicator. The aliquots were titrated against sodium hydroxide (NaOH) solution until end point was reached, after which volume of NaOH consumed was recorded. The value of the rate constant, k, obtained was 0.0002 s-1. The experiment was then repeated with 40/60 V/V isopropanol/water mixture and a larger value of k = 0.0007 s-1 was obtained. We concluded that the rate of hydrolysis of (CH3)3CCl is directly proportional to water content in the solvent mixture.