We then slowly added 25ml of chilled deionised water to the filtrate to initiate crystallization by using a measuring cylinder and a dropping pipette, once we had done this we left it for about 10 minutes to allow crystallization at room temperature. We then weighed a filter paper which we will use later in the experiment. We then collected the crystallized acetylsalicylic acid by vacuum filtration in a Buchner funnel and washed the product with a little ice-cold water. We then pre-weighed a clean, empty watch glass and labelled it with our initials and the date, we did this do we could easily identify that it was ours when we go to weigh it with the crystals on. We
Wash with 95% ethanol and wash with water after 15 seconds. 7. Stained counterstain with fuchsine and wash with water after 60 seconds. 8. Remove excess water from the surface of the gram stained glass slide and observed under 1000X oil immersion microscope.
Recovery broth was added to the cell suspension, and the bacteria was placed in warm water for about thirty minutes (see Results and Discussion, paragraph 2). This recovery period let the bacteria repair their cell membranes and express the added genes. Lastly, the transformed E. coli were placed on agar plates and allowed to grow overnight. One agar plate only contained nutrients (-DNA), two contained nutrients and ampicillin, (-DNA/Amp and +DNA/Amp), and one contained nutrients, ampicillin, and IPTG, a protein that caused the GFP to express a glow. After completing the lab, it was discovered that the ARG gene creates a resistance to antibiotics, like ampicillin, and that bacteria can take in new genes.
Crystal violet was then added for 60 seconds before being washed off with water. The mordant, Gram’s Iodine, was added for another 60 seconds before getting washed off with water. The heat fixed smear was then washed with 95% alcohol until the wash ran clear, leading to the final step of adding Safranin for 45 seconds before being rinsed with water. The slide was finally blot dyed with bibulous paper before it was placed under a microscope to observe the color and shape of the bacterium. 2.2 Litmus Milk Reaction A milk-based, litmus broth tube is incubated and observed after 48 hours.
A stir bar was also added to the solution. The glass stirring rod from previous steps was used to remove pieces of Cu that formed on the Al wire, so that more Al surface would be exposed. A steam bath was prepared with 50 mL of deionized water, the glass rod was used to remove as much copper from the aluminum wire as possible, and the Al wire was then disposed of in the solid waste container. The mass of an evaporating dish was recorded, and the Cu was transferred on to the evaporating dish. Water was removed from the dish, and the Cu was then washed thrice with 5 mL deionized water, and decanted between washings.
The solution was discarded into the waste bin, and the materials were washed. The second reaction in Part B, sodium hydroxide and ammonium chloride, began by saving the data from the first reaction and setting up the LabQuest to new data collection under the same conditions as the first reaction. The cups were restacked and placed in the beaker. Using a graduated cylinder, 50mL 2M NaOH was added to the cup. The cup was then covered and the temperature probe inserted.
Once the cola starts to boil, continue to boil it for another 10 minutes so that the carbon dioxide is removed. When the cola has finished boiling, cool it in an ice bath and pour the cola back in the volumetric flask and use distilled water to fill the flask to compensate for the evaporated water. Using a volumetric pipette, transfer 60ml of the cola to a beaker and put the magnetic stirrer in the beaker. Submerge the conductivity probe in the cola. Fill up the burette with NaOH
Lastly, it told us to repeat the same steps until we had three calcium chloride scoops in the beaker and repeat for two more trials for accurate results. To sum up the experiment, it said to record the average change in temperatures to the class averages to graph a bar graph comparing both of the averages. That’s the procedure on how to conduct the experiment correctly. The averages that my group received for zero scoops were 0.5 degrees Celsius, one scoop was 6.5 degrees
500gms of fresh beetroot or its hairy root has to be homogenized with sand continuously for three times in 70% ethanol (100mg/L).Then extracts will be centrifuged at 10xg for half an hour and supernatants will be allowed to vaporise at 40°C under vacuum till they get dried. Then, the residues will be dissolve in 0.5L of 70% of methanol and this sample-methanol mixtures keep in refrigerator at -20 degrees for 24hr, consequently thawed and supernatants are carefully collected from the precipitate. Under vacuum, methanol will be evaporated, at 40°C, from the supernatants and following betalains are lyophilized from aqueous fractions and finally 6.5gms of dry betalains will be obtained (Christ, Alpha 1-2,
Methanol was filled in a test tube and placed into a water bath to heat up. 12 Drops of the Methanol were then added to each flask until the crude caffeine had completely dissolved. 13. The solution was then filtered and the residue collected in a filter paper. It was left to dry and
Four randomly selected Daphnia magna, for each trial, were removed from the provided colony for the bioactive compounds to be tested, and were transferred with a plastic wide-mouth pipette with approximately 10 mL of pond water to protect and ensure survival of the Daphnia. In order to acclimatize the Daphnia to laboratory conditions, they were then placed onto a petri dish on the Daphnia cooling chamber. The cooling chamber was located on the stereomicroscope platform and brought down the heart rate of the Daphnia to a range that was countable by the observer, since Daphnia heart rate at room temperature is too rapid. On the cooling chamber there were two petri dishes: one for the Daphnia that were going to be tested, and one with the Daphnia being tested on, to ensure constant consistent temperatures for each trial. To maintain a temperature conducive to the heart
The liver removes the bilirubin from the blood and excretes it into the intestines as bile. When the liver is damaged, bilirubin, which is a yellow pigment, spills over into tissues and the blood, thus giving the skin a yellowish coloring. Jaundice is more apparent in the whites of the eyes. 4. Mrs. Fender’s prolonged clotting times and excessive bruising are related.
(1;p 326) Method I collected 1/8 c of saliva, then added the following: 2 drops of dish detergent (stirred 30 sec), 2 drops of contact lens solution (stirred 30 sec), and a small pinch of table salt (stirred 30 sec). Then, I tilted the cup approximately at a 45 degree angle and ran ¼ c of the ice cold rubbing alcohol down the side of the cup, which formed a layer on the top of the saliva-soap solution. (no mixing). Once the solution separated I then took two sticks and wrapped the DNA around them by gently spinning the sticks in the school with the DNA streaks. Final step was mailing the DNA sample to Tory Blackwell for PCR analysis.
This procedure occurred in the presence a Bunsen burner. The inoculation needle were placed within the open flame 15-20 second in order to sterilize the needle and prevent contamination. The needle was allowed to cool 5-10 second before inoculation. Using aseptic transfer technique the needle was used to gather up some of the colonies on the plate, making sure not to touch anything else with the needle. The test tube was uncapped and the lips of the test tube was passed through the open flame three times.