Comparing by DFA, there was wave number shifting occurrence to the lower wave number from 1693 cm-1 (DFA) to 1681 cm-1 (DFA-PRO). The change of the wave number of carbonyl group, indicated the hydrogen bond formation between DFA and PRO. This prediction was also supported by shifting of OH free carboxylic acid of DFA from 3324 cm-1 to 3270 cm-1. The new broad spectrums at 1905 cm-1 and 2541 cm-1 were generated from hydrogen bond of O...H-N (heterocyclic) or O-H...N (heterocyclic). This phenomenon was similar with cocrystal formation of diclofenac acid with pyridines and pyrimidines families (Aakeröy et al, 2011).
Because it is a tertiary benzylic halide, the reaction is considered an SN1 type. To test the purity, the class then uses a TLC. When one places,” a spot of the substance on the absorbent surface of the TLC plate, the solvent (or solvents) run up through the absorbent,” (Zubrick223). The initial mass of the reactant, triphenylmethyl chloride was 2.006 grams. The experiment yield is 1.589g, which is a 80.3% yield.
Top agar was dispensed into 50ml test tubes and then autoclaved for 15 minutes at 121℃ as well. Stored the top agar at -5℃ refrigerator when it cooled down. Preheated top agar for 20 minutes to liquefy before use. The optimal dispense temperature for top agar was 45℃. 3.3.4 Preparation of CaCl2 solution The molecular weight of CaCl2 was 111 g/mole.
The analysis was carried on C18 shim- pack GIST (150mmx 4.6mm 5µ) column used as stationary phase. A freshly prepared mobile phase consisting of methanol: potassium dihydrogen phosphate buffer in ratio of (30:70 v/v), PH-3 adjusted using ortho phosphoric acid (OPA) these were filtered by 0.45µM Whatmann filter paper and sonicated before use. The flow rate of mobile phase was 1ml/min. The detection was carried out at 220 nm and run time was around 10 minutes. Selection of wavelength A UV spectrum of drotaverine hydrochloride, ethamsylate, tranexamic acid in water was noted by scanning the solution in the range of 200-400nm.
The final volume was adjusted with the same solvent to get concentration of 100 µg/ml. the solution was further diluted to get the concentration of 10 µg/ml, filtered through 0.45 µm filter tips , and aliquots of 20 µl from this solution was injected into HPLC by using an
The most upfield of the carbons was at a PPM of 48 and belonged to the methyl carbon at the end of the ether substituent. A range of four carbon peaks falling between PPMs of 120-130 represented the benzyl compound of the methyl benzoate product. In part two of the lab methyl benzoate was subjected to a nitration resulting in the formation of methyl-3-nitrobenzoate. The purpose of part two was to add a nitrogen group to methyl benzoate by means of an electrophilic aromatic substitution (EAS) reaction. An EAS reaction pertains to the substitution of an aromatic hydrogen for an electrophile by means of an electrophilic attack on the aromatic ring which in this case is benzene.
Rose Bengal-(bis(aminoethyl)ethylene glycol) (2) from Rose Bengal disodium salt (1) The synthesis was done following procedure from . Rose Bengal Na+ salt (915 mg, 0.90 mmol) was dissolved in DMF (2ml) and DIPEA (0.312 ml, 1.80 mmol), HATU (308 mg, 0.81 mmol) were added. After activation for 15 min, the mixture was added to O-Bis-(aminoethyl)ethylene glycol trityl resin (309 mg, 0.31 mmol) preswollen in DMF for 2 hours. The coupling reaction wrapped in aluminum foil was allowed to proceed overnight on a nitrogen bubbler apparatus. The resulting red-burgundy coloured resin was filtered and washed well with DMF.
The solution homogeneity expelled, by centrifugation for 10 min. The sample was centrifuged and separated into two layers, and took the top of the sample is injected for HPLC (11,12). Measured concentration in total lipid: The total fats balanced concentration of the pesticide getting by dividing the measured pesticide residue concentration in the overalll tissue sample by the decimal fraction of the sample that consisted of ether-extractable lipid. The total lipid content of each specimen was estimated from its total cholesterol & triglycerides levels by using a summation method. Analytical results for organochlorine pesticides were reported on a lipid-adjusted basis (nanograms per gram or parts per billion) (14).
The mixture was incubated for 15 mins at room temperature and absorbance was measured at 510 nm. The total flavonoid content was expressed as quercetin equivalent (µg/mg of extract), which is a common reference standard. 2.4. DPPH radical scavenging assay The DPPH radical scavenging activity of methanol extract of leaves of Anisomeles malabarica was carried out according to the method .One mL of Plant extract of various concentrations (50-300 μg/mL) were mixed with 1 mL of 0.1 mM of DPPH solution in methanol. The reaction mixture was kept at room temperature for 30 min.
All standards, samples and solvents were filtered using filtered using 0.45 µm Sartorius Stedim, cellulose nitrate filter paper prior to HPLC-PAD analysis. The HPLC-PAD system consisted of a PAD detector, Perkin Elmer pump and ALS, CarbopakPA1 (4 × 250 mm) and CarbopakPA1 Guard Column (21.7 °C). The PAD detection range was set at 300 K (E1: 0.05 V, E2: 0.76 V, E3: -0.20 V). The injection volume used for analysis was 50 µL and the analysis time for each sample and standard was set at 30 minutes. The solvent system used for analysis was 10 mM NaOAc/ 150 mM NaOH and the kestoses were eluted at a flow rate of 1 mL /min.