Lastly, final concentrations and dilution factors were calculated by using the appropriate formulas. The final portion of the lab consisted of creating a lined scatterplot in Microsoft Excel with the absorbencies from the standard curve data chart. The chart was created to display the linear trendline, R-squared value, and slope equation. Then four sodas, Big Red, Big K Grape Soda, Faygo Red Pop, and Cherry 7 Up and one unknown sample containing red dye were processed through the absorbency tests, and diluted if necessary in a 1:1 or 1:3 ratio of water to soda. Using the equation determined from the standard curve graph, the concentrations of Red dye #40 was calculated for the sodas and the unknown liquid
The solution was filtered, diluted suitably and drug content was analyzed at 220nm using UV spectrophotometer and observations were recorded (table 1). Dissolution rate studies The dissolution studies were carried out using dissolution apparatus (Rotating Paddle type) at a speed of 50 rpm. Accurately weighed amount of drug, PMs and SDs immersed in a pH 2.0 HCl buffer as dissolution medium at 37±0.5oC. The dissolution was carried out for 1 h and aliquot of 5ml was withdrawn at adequate intervals. The filtered samples were suitably diluted, assayed at 220nm and cumulative percentage of the drug dissolved from the formulations was calculated (table 2,
3- Put the large tube in an ice bath and start to add alcohol by an increasing rate . 4- Put the tube in hot water (70º C) for 5 minutes . 5- Add 8 drops of water with constant shaking then leave the reaction to cool to room temprature. 6- Add 6 mL of ( 3 mL saturated NaCl +3 mL distilled water) i.e : half-saturated sodium chloride solution to the test tube with vigorous shaking and then wait until two layers form (upper layer is ester while lower layer which consists of a water solution of sulfuric and acetic acids ,should be discarded
Acetonitrile at a PH of 7 (neutral) is added to each of the test tube samples. Mix the samples on a vertex shaker for 3 minutes and transfer to a 20 ml centrifuge tube and place in a TurboVap under 5-psi nitrogen at room temperature and allow it to completely dry. The dry resides are now put in 1ml of acetonitrile for testing (analysis). 4. Chromatographic Condition 10ml of the extract is now taken to be analyzed using a mass spectrometer and a liquid chromatograph.
Introduction In a two-week laboratory experiment my group and I used the techniques of acid-base extraction and Thin Layer Chromatography (TLC) to extract the three active compounds in Excedrin- aspirin, acetaminophen, and caffeine- and confirm the identity of our extracted crystals. The purpose of this experiment is to introduce organic chemistry lab students to the laboratory techniques of acid-base extraction and TLC. This lab also offers the opportunity to apply the previously theoretical classroom concepts of acidity, solubility, and polarity in order to utilize acid-base extraction and TLC. The aim of this experiment is to take an Excedrin tablet and use the varying acidities of the 3 active components to separate them using acid-base
The vacuum pump was switched on to suck the evaporated volatile solvent. After 30 minutes of evaporation, the heating system was switched off and left to cool down for another 30 minutes. Finally, the flask was weighed and the concentrated extract was taken out. 3.5.1
1.25mL of acetic acid and ferric chloride mixture was added to 2.5mL of the pure extract. Occurrence of blue-green color indicates the presence of glycosides. Test for Saponins. 10mL of distilled water was added to 5 mL of the pure extract the mixture was shaken in a graduated cylinder for 15minutes. Occurrence of layer of foam or bubbles indicates presence of saponins.
Briefly, a solution containing 0.3 gr (3 mmol) succinic anhydride and 0.4 ml (3 mmol) of triethylamine in 10 ml of THF was dropwise added to a stirred solution of 1 mmol of sPEG in 10 ml of anhydrous THF for 12 h at 75 C. The solvent of product solution was evaporated by a rotary evaporator and the obtained dark yellow viscous liquid was dissolved in acidic water (pH= 3). In the following,
HPLC-grade methanol (15 mL) was added to a quantity of the powdered tablets equivalent to one tablet. This solution is sonicated for 20 min. Filtration through Whatman No. 1 filter paper followed by quantitative transfer of the filtered solution into 25 mL volumetric flask and then dilution was made to volume with methanol. A further dilution was made to reach final concentration of 102.8 µg/mL, 97.2 µg/mL for VAL and SAC respectively.