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Stock Concentration Lab Report

293 Words2 Pages

The concentration of the stock solution 2.0x 10-4M as per label information in the lab. However, the calculated volume using the experimental data is 1.5 x 10-4M.There is 25% difference between these concentration caluclated from zero time intercept.The significant difference in the concentration drop happened by many factors.First,the rate ionization is depend the pH because pKa determines the equlibrium between p-nitrophenol and its depronated form p-nitrophenolate.Although,the pH is maintained by buffer at 7,not all of the p-nitrophenol produced by the chymotrpsin catalysis is not its depronated form.The concentration calculation is based on the absorbtion which depends on the p-nitrophenolate form.This could have contributed to the drop

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