Abstract: The purpose of this experiment was to identify given Unknown White Compound by conducting various test and learning how to use lab techniques. Tests that are used during this experiment were a flame test, ion test, pH test, and conductivity test. The results drawn from these tests confirmed the identity of the Unknown White Compound to be sodium acetate (NaC2H3O2) because there were no presence of ions and sodium has a strong persistent orange color. The compound then will be synthesized with the compounds Na2CO3 and HC2H3O2 to find percent yield. Weighed 1 gram of NaC2H3O2 and mixed it with ionized water.
Standardization of NaOH solution Accurately weigh out a sample of approximately 0.3-0.4 g of primary standard potassium hydrogen phthalate, KHPh, which has been previously dried at 120°C. Do not use more than 0.4 g. To obtain an accurate mass, weigh the sample on weighing paper, slide it into a clean (but not necessarily dry) 250 mL Erlenmeyer flask and reweigh the paper to account for any KHPh that may remain on it. Dissolve the KHPh sample in about 50 mL of CO2-free water and add 2-3 drops of 0.1% phenolphthalein indicator. Begin adding the approximately 0.1 M sodium hydroxide solution from the buret while continuously swirling the flask contents. Do not open the stopcock completely.
(ii) Acidify to methyl orange end point with concentrated H2SO4. (iii) Add 5 ml concentrated HNO3. (iv) Add 2 ml 30% H2O2 . (v) Evaporate on a hot plate to 15 to 20 ml. (vi) Transfer the concentrate and any precipitate to a 250 ml conical flask using 5 ml concentrated HNO3.
Calibrate the spectrophotometer the same exact way so that it does not mess up the calculations gathered. This week using yellow dye #5 mix .20 milligrams of this will 250 ml of distilled water in a volumetric flask. Make five different solutions, distilling the water each time, of new yellow dye solution and place each of the solutions in a blank cuvette to find the max absorbency of each the solutions. Next during week two the absorbency had to be found from the food that was brought in. Using a blank cuvette place the commercial liquid and calculate the concentration and absorbency and which dyes would
The Swirling flask test uses 120 ml of seawater and 0.1 ml of oil. The 0.1 ml oil added into 120 ml of seawater in a modified 125 ml Erlenmeyer flask with side spout. Dispersant is added onto the oil surface floating on water surface. Another variant is to premix the dispersant with oil and adding them to water (Sorial, et al., 2001). Then, the flask is put on shaker table and mixed at 150 rounds per minute before allowing them to settle for 10 minutes.
Each 0.1M Sodium hydroxide solution that had been rinsed was drain into the waste container located under the hood. 3. The burette was filled with 0.1M Sodium hydroxide solution(prepared prior of this experiment) to 50 ml volume and the burette was clamp vertically(the air bubbles was remove from the tip of the burette by draining the 0.1M Sodium hydroxide into smaller beaker) 4. The 10g or 10ml amount of samples was inserted into 250ml conical flask and with addition of 50ml distilled water 5. Three to five drops of phenolphthalein were added into conical flask 6.
Stored in light glass bottle. 2.5.2: Formation of PANI(CoFe2O4) 0.1g of Nano material and 50ml of Aniline hydrochloride were mixed in a beaker. Then 50ml of Ammonium peroxydisulphate was added drop wise in reaction mixture with constant stirring below 20 oC. After 24 hours the coated sample was filtered, washed and dried at 60 oC in oven and then grinded into a fine powder in agate
1. Label each well of a tissue culture treated 6-well plate appropriately for each cell line or condition being investigated. 2. Prepare 2x cell culture medium by dissolving 1 g of powder medium and 0.2 g of sodium bicarbonate in de-ionized water to a final volume of 50 ml. 3.
Heat the Agarose gel in a 65 °C water-bath to melt the agarose. After it melted, maintain its temperature at 55 °C until it is ready for use. 2. Transfer the spheroids from the 96-well plate to a 15 mL centrifugal tube using a 1000 μL pipette 3. centrifuge the tube for 5 min at 1000 rpm to form a pellet. 4.
It was then ashed with a muffle furnace at 500ºC and weighed (W3) . The percentage of ash was calculated by using the following formula: Ash (%)= W3-W1 ̸ W2-W1 X 100 Ash was treated with conc.Hcl and was made upto 50ml with distilled water. An aliquot of ash solution was reacted with ammonium oxalate solution for calcium precipitation. After centrifugation and decanting supernatent, the precipitate is redissolved in 4N sulphuric acid. Calcium solution is titrated against 0.01N Potassium permanganate to obtain pink colour as end point.