Red Strawberry Fruits

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A] ANTIOXIDANT ASSAYS In the past decade, strong attention was given to the antioxidant power of fruits as an indicator of its quality. This parameter is strictly proportional to the presence of efficient reactive-oxygen/radical scavenging phenolic compounds and other secondary metabolites. Highly reactive molecules (NO, O2-, HOO-) generated in biological systems are associated with initiation of degenerative diseases. The antioxidant can reduced this risk. Uncontrolled generation of free radicals together with reduced level of antioxidative vitamins and enzymes are considered to be main contributor to oxidative stress (Ellnain-Wojtaszek et. al., 2003). Free radical attack membrane lipid, protein and DNA which is believed to be involved in …show more content…

Chemical profiling of fruits of both the varieties from four different ripening stages was performed using high-performance liquid chromatography (HPLC). Chlorogenic acid, Catechin, Rutin and Quercetin were used as standards. The peaks obtained in various chromatographs were compared with the standards. Although HPLC data is available for red strawberry fruits, no such information has been reported for the two strawberry varieties we assessed. Further, only a couple of reports are available on the HPLC analysis of fruits from four different developmental stages (Zhang et al. 2011). There are a few reports on HPLC analysis of strawberries (Aaby et al. 2007; Buendia et al. 2010). From Figures 5, 6, 7 and 8, we determined that more compounds were observed in 0.2% acetic acid extract which were eluted at 280 nm than the ethanol extract. Additionally, in the antioxidant assays described above, more activity was seen in the 0.2 % acetic acid extracts except for the ferric reduction potential assay. A recent study revealed significant changes in metabolite levels during the ripening stages, specially the transition stage from green to red (Zhang et al. 2011). Though reported for the first time in Sweet Charlie and Camarosa varieties, our HPLC data is similar to what was reported for other varieties (Zhang et al.

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