Substrate Concentration Lab Report

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Hypothesis: Increasing substrate concentration will increase the initial reaction rate until it stops increasing and flattens out.
Independent Variable: Substrate concentration
Dependent Variable: The substrate itself, 1.0% Hydrogen Peroxide
How Dependent Variable will be Measured: Hydrogen Peroxide will be used in every experiment, just with different test tubes. The amount of Hydrogen Peroxide in the mixing table is the amount that will be added to each test tube.
Rationale: Enzymes are used to make reactions faster. When there is more substrate the enzyme will be forced to work harder to get rid of it faster. The problem is that eventually the enzyme hits a threshold where it becomes so saturated with substrate that it can’t go any faster,

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