So, each potato cylinders may have contained different amount of sugar thus affecting the rate of osmosis. Also, when time elapsed and we came to take the potato out of the test tube it took time as we had variety of trials therefore increasing unreliability of results since some potatoes remained in the test tube and exceeded the time in which it had to stay
The mass of the before the experiment was ranged from 8.520 g to 18.46g. The weight between each of the potato was different because our goal was to find the percent change in mass, which made the mass of the potato don’t have to be the same. The mass after the experiment was ranged from 7.280g to 15.05g. We could tell the solution concentration in 0.0M, and 0.2M make the potato’s mass increased which making the potato hypotonic. The mass decreased in the solutions of 0.4M, 0.6M, 0.8M, and 1M making them hypertonic.
By using the same mass of potato slices and putting them in different concentration of solutions for a specific amount of time will tell us how the concentration changes the mass of the potato slice. Therefore changing the rate of osmosis. Hypothesis: I predict that, if the piece of potato was put into a solution that has a high concretion of sucrose then the potato slice would lose mass as it would lose water from its cells because the water is moving out of the cell from a high concentration to a low concentration of water through a semi- permeable membrane. The cell is hypotonic and the solution is hypertonic. Plasmolysis takes place as the cell loses water.
In a big pot, put a gallon of water and 2 tablespoon of salt to a boil over high heat. You may want to peel the potatoes or leave the skin on for a rustic feel. Peel it and slice them into uniform pieces. This will make sure that it will all be cooked at the same time. Keep them in a cold water until they are ready to cook, you can do this for up to 4 hours in advance before boiling the water.
After they were cut into 2.00 cm each we found the mass. We zeroed out the scale and weighed all four potato cores at once and recorded the mass. We then put those potato cores into the beaker of 75 mL of solution. With the potato cores in the beaker we then put a watch glass over the top of the beaker to minimize the amount of solution that evaporates. We let the potato cores sit in the solution overnight.
Research Question What impact will solutions of different concentration have on the percentage change in the masses of potato tubes? Variables Independent Variables The concentrations of sucrose in the solution (M per mL): 0.2 0.4 0.6 0.8 1.0 The control: Water with no salt/sugar added (therefore 0.0 M per mL) Dependent Variables: The percentage change in the mass of the potato tubes after bathing them in sucrose solutions of different concentrations Controlled Variables The variable How it was maintained Potato tubes By using the same potato to cut out all the potato tubes By using a potato corer of the diameter of 5mm By cutting the potato tubes into 3 cm each using the same ruler Water used By using the water samples
There group of rats were used in our study (n= 6–8 rats/group) as follows Control-group received vehicle (corn oil daily) for 2 weeks, VPA-group received VPA alone (500 mg/kg PO, daily) for two weeks and VPA+DHA-group received VPA (500 mg/kg PO, daily), followed by DHA (250 mg/kg PO, daily) for two weeks. After two weeks of treatment, rats were terminated using sodium pentobarbital (50 mg/kg, IP) for liver collection. Liver was isolated, weighed, aliguoted in few tubes and snap frozen in liquid nitrogen. A 10 % (w/v) liver homogenate was made in phosphate-buffered saline (PBS) (pH 7.4) for the assay of hepatic TBARs, NADPH, NrF2, and HO-1. Other frozen liver samples were homogenized in RIPA buffer for western blotting (n=4/group).
H and S were diluted 20x and P1 and P2 were diluted 2x to make up a total volume of 1ml each. 50ul of each diluted sample was pipetted into 8 wells of the microplate and 50ul of each protein standard was pipetted into 2 wells. 50ul were incubated with 50ul of alkaline copper reagent. 50ul of alkaline copper reagent was added to every well containing water, buffer, sample or standard and was incubated for 30 minutes. 100ul of Folin reagent was added to the wells and incubated for another 20 minutes.
Mix until just combined. Scrape the dough out of the mixer bowl directly onto plastic wrap. Wrap it up tightly and refrigerate for 20 minutes. Once your dough is chilled, you can roll it out and start cutting some donuts! Roll to 1/2" thick and go to town with a 3" round cutter and 3/4" hole cutter out of your Round Cutter Set.