Isolation Of Plant Extract Essay

3. To isolate plant extract from leaf, fruit and stem of Aegle marmelos and Terminalia belerica.

3.1 Extraction of the Aegle marmelos plant material

Here hot solvent extraction process was used for extraction of the plant material. Four solvents were used for the extraction of the plant material. They were as follows

i) N hexane ii) Petroleum ether iii) Methanol iv) Chloroform

The powdered plant material (50 gm) was successively extracted in a Soxhlet extractor with an elevated temperature using 250 ml of n-hexane, followed by petroleum ether, methanol and chloroform, according to increasing solubility. All extracts were filtered individually and poured on petri dishes to evaporate the solvents from the extracted material. After drying, crude

Four solvents were used for the extraction of the plant material. They were as follows

i) N hexane ii) Petroleum ether iii) Methanol iv) Chloroform

The powdered plant material (50 gm) was successively extracted in a Soxhlet extractor with an elevated temperature using 250 ml of n-hexane, followed by petroleum ether, methanol and chloroform, according to increasing solubility. All extracts were filtered individually and poured on petri dishes to evaporate the solvents from the extracted material. After drying, crude extracts were stored in stock vials and kept in refrigerator for further use.

3.3 Qualitative chemical examination of extracts

Detection of alkaloids

Extracts were dissolved individually in dilute hydrochloric acid and filtered. The filterates were used to test for the presence of alkaloids.

Mayer’s Test

Filterates were treated with Mayer’s reagent (saturated solution of potassium mercuric iodide). Formation of a yellow cream precipitate indicates the presence of alkaloids.

Dragendorff’s test

Filterates were treated with Dragendorff’s reagent (saturated solution of potassium bismuth iodide). Formation of red precipitate indicates the presence of