Summary Of Gum Prunus Cerasoides Gum
2.1. Materials and Methods The gum Prunus Cerasoides D.Don were collected from khawzawl , Mizoram, India, during the month of May-July, India. Diclofenac sodium (DS) was gifted by Kowsik Pharmaceuticals, Chennai, India. Sodium alginate (SA) and Calcium chloride were purchased from SD Fine Chemicals Limited, Mumbai, India. In this study all chemicals and reagents were used analytical grade. 2.2. Purification Method for Prunus Cerasoides Gum The PC crude powdered gum was dissolved and boiled using 80 % ethanol solution to deactivate enzyme and remove low molecular weight carbohydrates and coloring matter. Thereafter, it was dispensed with sufficient quantity of deionized water and stirring properly throughout the night using a magnetic stirrer. Then gum solution was kept to allow without disturbance up to 12 h at room temperature to detach any undissolved matter. Followed by the PC gum solution was filtered by triple folded muslin cloth and evaporated up to one third of original volume using a rotary vacuum evaporator then cooled and precipitated with isopropanol and air dried. The end product was passed via sieve (no. 85) and stored in desiccators whenever the PC gum was used. 2.3. Preparation procedure for DS loaded PC-SA combined beads The ionotropic gelation method was used for the preparation of DS loaded PC-SA bead. The gum (PC) was dissolved in distilled water and initially boiled for 10 minutes, then cool and keep stirring for 24 hours at 400 RPM. Then filtration
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