There is a significant difference among the motor oil subjected with increasing concentration of bromelain enzymes in terms of decomposition rate. Chapter 3 METHODS Bromelain is a sulfur-containing proteolytic digestive enzyme that is extracated from the stem and the product of the pineapple plant (Ananas comosus, family Bromeliaceae). At the point when brought with meals, bromelain is believed to aid the processing of proteins. At the point when taken against a void stomach, it is accepted to act to act medicinally as an
1. Sample Collection Fermented milk products i.e. curd and lassi samples were purchased from a local dairy for isolation of lactic acid bacteria. Spoilt fruit samples were collected from local fruit market for isolation of spoilage-causing organisms. Good quality pineapples were purchased from the same fruit market for the preparation of juice.
What pH range is required for jams to set or gel? Pectin causes the jelly or jam to set and the bonds that it creates resulting in a gel are strongest at a pH of 2-8 to 3.2 (Herbstreith & Fox n.d.). 3. Why are moulds a problem in jams and jellies? The air within the jam jar
This experiment was done by using a mandarin orange. First, a mandarin orange was peeled off and squeezed to get 30ml of its juice in a beaker. Next, the juice was diluted with distilled water to get a measured volume of 50ml solution. After dilution, the solution was transferred into a 100ml Erlenmeyer flask. As it was done in the Experiment A, 20 drops of 0.2 M acetic acid and 10 drops of 2% starch solution was mixed well with the juice solution.
TLC method 1 Solvent system: Chloroform: methanol: 25% ammonia (8:2:0.5). Spots can be detected after spraying with Dragendorff reagent Orange spot (Mallikharjuna et al., 2007). 2) Anthraquinone Borntrager's test Heat about 50mg of extract with 1ml 10% ferric chloride solution and 1ml of concentrated hydrochloric acid. Cool the extract and filter. Shake the filtrate with equal amount of diethyl ether.
Ascorbic acid (as the reference standard) and the sample were reacted with DPPH solution in methanol/water for four hours at 35°C in a vessel mounted on a rotary shaker and the absorbance changes were measured at 517 nm. The quantity of sample necessary to react with one half of the DPPH is expressed in terms of the relative amount of ascorbic acid reacted. Antioxidant activity of a sample is expressed in terms of micromole equivalents of ascorbic acid per 100 grams of sample, or simply ascorbic acid units per 100
Following are the steps involved in fermentation of cucumber pickling. • Harvesting • Washing • Brining • Fermentation Harvesting: • In this method cucumbers are poured into a jar or tank and then brine solution is poured onto the cucumbers so fermentation is allowed to proceeds. Fermentation take at 18-20 C and a naturally occurring bacteria (lactobacilli) present on the cucumbers. In either case, the bacteria are halophyllic, or salt tolerant. During a storage period of about five weeks, the fermentation bacteria breakdown the sugars present in the vegetable and produce carbon dioxide.
The peel powder was soaked overnight (or for desired period) at room temperature (30-32°C) for extraction with intermittent shaking. After extraction, it was centrifuged at 2000 rpm for 5 min and filtered through Whatman No. 2 filter paper. The effective volume obtained after centrifugation was noted. 2.3 Determination of radical scavenging activity (RSA) Determination of RSA was carried out according to Murthy, Jayaprakasha, & Singh (2002) using DPPH as stable free radical and butylatedhydroxyanisole (BHA) as standard.
Comparative analysis of pectin extraction from Pomegranate and Sweet Lime peels Dr. Shoba Ganesh and Ganesh Kumar Abstract: Conventional Soxhlet Extraction methods have been used to extract pectin from pomegranate and sweet lime peels, with different extraction periods, solvent pH and temperatures, to detect the optimum conditions for maximum pectin production. Tests were also done to study the chemical properties like Equivalent weight, Methoxyl content, Galacturonic Acid content and degree of Esterification. Pectin extraction has been done keeping the peels to solvent ratio of 1:5 initially. The pectin yield from pomegranate peels was higher (24.6%) compared to that of sweet lime peels (21.6%). The maximum equivalent weights from sweet lime and pomegranate peels extracted pectin samples were 2083.33 and 14,285.71 respectively.
Four solvents were used for the extraction of the plant material. They were as follows i) N hexane ii) Petroleum ether iii) Methanol iv) Chloroform The powdered plant material (50 gm) was successively extracted in a Soxhlet extractor with an elevated temperature using 250 ml of n-hexane, followed by petroleum ether, methanol and chloroform, according to increasing solubility. All extracts were filtered individually and poured on petri dishes to evaporate the solvents from the extracted material. After drying, crude