Comparing The Separation Of Pineapple-Carrot Oranges

2.2 Preparation of Pineapple-Carrot-Orange-HSE beverage formulations
Mature pineapples, carrots and oranges were obtained from a local market near the Federal University of Technology, Akure. Pineapples, carrots and oranges were sorted, washed and peeled. Pineapple and carrot juices were obtained using a champion juice extractor, model number: KP60PD (Hallelujah Acres, Shelby, North Carolina). The orange juice was obtained using a manual juice extractor. Dirt and other foreign materials were removed from the Roselle calyces by hand picking and winnowing. The Roselle calyces were then crushed and extracted using the ratio calyces: hot water, 50g: 1000L for 15 min. Extracts were further clarified using a sterilized cheese cloth. Beverage formulations

An aliquot of 2.5ml was mixed with 2.5 ml of 200 mM sodium phosphate buffer (pH 6.6) and 2.5 ml of 1% potassium ferricyanide. The mixture was incubated at 50oC for 20 minutes and then 2.5 ml of 10% trichloroacetic acid was added. This mixture was centrifuged at 800 × g for 10 min. An aliquot of 5 mL of the supernatant was mixed with an equal volume of water and 1 ml of 0.1% ferric chloride. The absorbance was measured at 700 nm and ferric reducing power was subsequently calculated using ascorbic acid

The non-linear presentation of the contours indicate a strong interaction between the design variables. The ability of the formulations to scavenge the radical increased with increasing amounts of pineapple, carrot, orange and HSE.
DPPH=206.90A+140.93B+335.21C+73.19D-325.10AB-909.49AC-283.55AD-728.4BC-130.13BD-542.09CD+374.21ABC+16.24 ABD+908.48 ACD+760.05BCD……………… 2 where A= pineapple , B= carrot, C= orange and D=HSE. The R-square value = 0.9787, Adjusted R-square value = 0.9326
The beverage formulations neutralized the DPPH radical which has a deep violet colour in solution, and it became colorless or pale yellow. This property allowed visual monitoring of the reaction recorded as % DPPH inhibiting capacity (Sharma and Bhat,