In this multi-step experiment, there were multiple hypotheses which were stated. The first hypothesis was that if an enzyme catalase is added to Hydrogen Peroxide, then the temperature of the H2O2 will increase. The other two pertained to an enzyme being affected. If boiled catalase is mixed with Hydrogen Peroxide, then there will be a larger temperature jump than if only pure catalase was added. The third hypothesis was if catalase with acid is added to H2O2, then the temperature will have a smaller jump than if only pure catalase was added. The above hypotheses were partially correct and partially incorrect. After adding catalase to Hydrogen Peroxide (HP), the temperature did increase rapidly, but then started to decrease in temperature. The second and third hypotheses were incorrect. In both cases, the temperature rose under a degree or did not rise at all, which contradicted the hypotheses. …show more content…
The multiple experimental groups consisted of HP mixed with either normal enzyme catalase, catalase with acid, or boiled catalase. The pure catalase was largely different than the other two “affected” catalase, as it jumped from 23.5 °C to 33°C in one minute. After the the jump, it decreased at 0.5°C per minute, finally ending at 30°C. The other two experiments had mostly the same effect. The boiled catalase jumped 0.2 degrees in 30 seconds, then stayed for the rest of the time. The acid catalase remained at 23.5 degrees during the entire test, just as the pure H2O2
How Temperature Effects the Catalytic Ability of Peroxidase from Potatoes Abstract In order to determine if temperature affects the ability of Peroxidase to react, we measured the reaction rate of the same solution exposed to different temperatures. Solutions were exposed to a 4-degree, 22-degree, 32-degree, or 60-degree Celsius environment then measured by a spectrophotometer. The solution left in the 22-degree environment had the highest reaction rate, while the solution exposed to the 60-degree water bath was not able to react at all.
Nevertheless, the effects caused by the breakage of bonds will eventually lead to a decrease in the rate of reaction. As seen in the data, the reaction rate increased from 0.088 to 0.101 throughout the interval of -5℃ to 20℃ then decreased to 0.037 throughout the interval 20℃ to 56℃. This can be explained by the fact that 20℃ is the optimal temperature, therefore the active site of the enzyme is complementary to the substrate, causing the rate of reaction to be
Catalase Activity on Substrate Based On Gas Pressure Production Rate Name of the Class Author’s Name Date Enzymes are organic compounds which act as catalysts and speed up biological reactions in biological organisms. They are not destroyed or changed during the reaction but rather they are used over and over again to catalyze many more reactions. Their activity may be affected and altered by factors such as temperature, substrate concentration, enzyme concentration and Ph.
Title: Enzymes Abstract: Enzymes can catalyze chemical reactions by speeding up the chemicals activation energy. Temperature and pH are just two of the factors that affects enzymes and their involvement with chemicals and the way they function. Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. The following information consist of the recordings of when it was exposed to four different pH levels to come up with an optimum pH and IRV at the end. Introduction: Enzymes are proteins that are used in reactions in living organisms.
7. Will the temperature effect the pH Scale? Hypothesis: The hypothesis of this experiment is that the rate of reaction will increases well as the hydrogen peroxide concentration. If the temperature, pH and enzyme concentration is kept constant then the rate of reaction will start to decrease as well as the hydrogen peroxide concentration. Aim: To investigate the effects of changing the concentration of the enzyme catalase that it has on the rate of breaking down the Hydrogen Peroxide solution.
The lab results would have been more accurate if they were based off quantitative properties instead. For instance, if the reaction between catalase and hydrogen peroxide was timed using a stopwatch to see how long the oxygen gas bubbled for, the times could have been compared to obtain a more accurate reaction rate for each reaction. Furthermore, the temperature of the solution after the test tubes were taken out of their respective water baths were not monitored. This could have been a factor that affected the reaction rate of catalase, since the reactions may have not occurred at the same temperature. A thermometer could have been used to measure the temperature of the contents in the test tubes once they were removed from the water baths to ensure that all the reactions took place at the same temperature as the baths they were placed
It was hypothesized that the optimal pH for the enzyme was pH 7 while the 1.0 ml peroxidase would have the best reaction rate. At the end of the experiment the results prove the hypothesis to be incorrect. INTRODUCTION Enzymes are proteins that allow a reaction to speed up. These proteins are made up of monomers known as amino acids.
The addition of the extra catechol was supposed to have sped up the reaction with the enzyme and force the inhibitor out of the enzymes active site. If there was more time allowed to observe any possible color changes the results would have been more conclusive and our results more accurate. In other
The objective of this lab was to determine the best pH level to increase enzyme activity. As this objective was met, it was discovered that water (pH level 7) was the best for percent absorbance. The hypothesis for this experiment was, “If peroxidase is an enzyme and therefore contains certain pH tolerances, then when placed in solution with pH levels of three, seven, and ten and the reaction is measured by a colorimeter, then water will be the optimal solution for maximum reaction rate.” As seen in the tables and graphs, the data supported the hypothesis due to the fact that most enzymes have an optimal pH of 4-9.
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.
purpose the propose of this experiment was too see if the chemical reaction of a enzyme can be made faster. Hypothesis I think that a warm environment would be best to make an enzyme’s reaction faster. because a protein can move faster in heat.
Introduction In class, a series of experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas. Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were formed.
Most enzymes work best at body temperature of 37 (degrees Celsius). PH is another factor that affect the rate of enzymes activity. Enzymes function slowly in lower pH like in the pH of 1 or 2. Potato are high catalase when is being placed in a pH of 2 solution and hydrogen peroxide is added to it there will be some fizzing but not a good one. If the potato is placed in a pH of 13 and hydrogen peroxide is added there wouldn?t be any fizzing or little fizzing meaning that high pH either denature the catalase or substrate can not bind to the active site of an enzymes because its changes its shape.
5 water bath were set up each to10 °C. (5 were used do the experiment faster) 5 cm3 of starch solution were added into the 5 test tubes that were labeled test tubes. Then 5 cm3 of amylase enzyme was added into the other 5 test tubes that were labeled. Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C).
ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.