Tannase Lab Report

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S.K.Mohan, T.Viruthagiri and C.Arunkumar

Kinetic Studies on Microbial Production of Tannase from Redgram husk
Abstract — Tannase (tannin acyl hydrolase, E.C.3.1.1.20) is an important hydrolysable enzyme with innumerable applications and industrial potential. In the present study, a kinetic model has been developed for the batch fermentation used for the production of tannase by A.flavus MTCC 3783. Maximum tannase activity of 143.30 U/ml was obtained at 96 hours under optimum operating conditions at 35oC, an initial pH of 5.5 and with an inducer tannic acid concentration of 3% (w/v) for a fermentation period of 120 hours. The biomass concentration reaches a maximum of 6.62 g/l at 96 hours and further there was no increase in biomass concentration
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From plant sources, the enzyme is present in tannin rich vegetables mainly in their fruits, leaves, branches and barks of trees like konnam, mirobolano and badul [8]. Many fungal species have been proved for its ability to produce tannase that have higher enzyme activity compared to bacterial and yeast tannase. The most important source to obtain the enzyme is by microbial way, because the produced enzymes are more stable than similar ones obtained from other sources [7]. In this study, the kinetics of tannase production using A.flavus with redgram husk was studied and various unstructured kinetic models were used to characterize the fermentation…show more content…
The samples were drawn at regular time intervals and analyzed for tannase activity, biomass concentration and residual substrate concentration and the results are shown in Fig.1.The tannase activity was found to increase with respect to fermentation time and reaches a maximum of 143.30 U/ml at the end of 96 hours of fermentation and then decreases gradually till the end of fermentation. The biomass concentration reaches a maximum of 6.62 g/l at 96 hours and further there was no increase in biomass concentration till the end of the

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