The line of best fit gives the respiration rate of day-old seedlings as the concentration of NaCl they are exposed to increases. As NaCl Concentration increases the rate of cellular respiration decreases by .108 ppm CO2/g per second. This overall decrease throughout the data further supports our hypothesis. Discusion: The data collected in the experiment does support our hypothesis. By examining the data as a whole a trend of decreased cellular respiration in seedlings soaked in solutions with increased NaCl concentrations.
(see table #2) The mixture with the bean water caused the solution to not be as concentrated, limiting the amount of oligosaccharides that the alpha galactosidase can break down, therefore resulting in a small amount of glucose concentration. The highest stand standard deviation is at 4 mL of alpha galactosidase, which is 185.742. The lowest standard deviation is at 0 mL and 1 mL of alpha galactosidase, which is 0. Since error bars are not all overlapping, it shows that there was a significant difference (see figure #3). However, the R squared value is 0.929, meaning that it is close to fitting the line of best fit.
The three things that can cause the enzyme to denature is a large change in pH level, High Temperature, and substrate concentration. According to our knowledge, we know that a large change in pH will cause instability in the protein structure thus resulting in denaturation of the enzyme. From the data, we can see that pH 3 (total:6.3) and 10 (total:6.2) were the slowest because pH 3 is probably the highest acid and pH 10 is the highest base. The highest acid or base pH represents a large change which would cause the enzyme to denature. The fastest pH was 6 (total:34.5), and it seems that there wasn’t a large change which resulted in a stable structure.
On the other hand, 132 seconds were needed for the solution with the crushed tablet to stop reacting, which is the one with the reactant having a larger surface area. In a reaction, the absence of fizzing indicates that the chemical process has ended. Average rate of reaction (Whole Tablet) = 100 / 160 = 0,625 Average rate of reaction (Crushed Tablet) = 100 / 132 = 0,757 0,757 > 0,625 All these information signifies that the powdered vitamin C increased the reaction rate by causing the reaction to take place in a decreased amount of time. From the graph it is also clear that the rate of reaction took place with a fairly higher speed when the powdered vitamin C was used. Based on the previous results, the hypothesis was confirmed.
The aim of the investigation was to determine the effect of pH on the rate at which catalase decomposes hydrogen peroxide and consequently answer the researchable question “How does pH influence cells and consequently an organism”. The hypothesis, “As the pH deviates from 7 the initial rate of oxygen production will decrease” is supported by the results. The trend displayed in Figure 3 is, as the pH deviates from 7 the initial rate of reaction decreases. Figure 3 shows that the rate of reaction (%O2/s) decreases substantially as the pH increases and decreases to 8 and 6 respectively from a pH of 7. This is indicated as the graph shows that the initial reaction rate for pH 7 was 0.143 %O2/s compared to 0.047 and 0.053 for pH 6 and 8 respectively.
Fig. 6 (a) shows the effect of hydrogen peroxide increase on the MB removal at constant pH 3 and Fe3+ of 40 mg/L. The results show that the degradation rate of MB increases with an increase in initial H2O2 concentration from 100 to 400 mg/L, but in excess of about 400 mg/L; the H2O2 dose of 1000 mg/L, plot of the reaction rate curve is almost horizontal. This could be illustrated that the presence of H2O2 beyond the ratio with Fe3+ does not improve the MB degradation. According to Murry and Parson (2004) hydrogen
paracasei isolated from the Portuguese dairy industry was found to be 2.5 mg/ml with surface tension 41.8 mN/m (Gudina et al., 2010). Those surfactants can decrease the surface tension of water or PBS around 36-39 mN/m and even their cmc values ranges from 1.0-20 mg/mL. As compare to the chemical surfactant surface tension reduction and cmc values it has been very close to the surface tension reduce by the SDS (Sodium dodecyl sulfate) i.e. 37.0 mN/m and 1.8 mg/L respectively. Biosurfactant produced by the Lactobacillus spp.
But there is a very important fact about blood alcohol concentration is that when the amount of alcohol in the body increases , the proportion eliminated in an hour decreases. Let P be the proportion of alcohol broken down in a given hour and a be the number of grams of alcohol in the body at the beginning of the hour then P = 10/(4.2+a) This is called Capacity-limited
The quantity of acid neutralised is obtained by subtracting the moles of acid given at the start of the experiment, with the moles of acid titrated. Back titration is used for this experiment as Calcium Carbonate is volatile and is an insoluble salt. Therefore they cannot fully dilute with water. As it’s chemical and physical properties are non ideal for a Direct titration, Direct titration cannot be used to determine the Calcium Carbonate content in toothpaste. In Back titration, excess volumes of HCl acids are added to allow for titration with NaOH.
The mobile phase used was a mixture of ammonium acetate buffer and acetonitrile at a ratio of 400:600. A flow rate of 1 mL/min was maintained, and the detection wavelength was 292 nm (22). The required studies were carried out to estimate the precision and accuracy of the HPLC method and were found to be within limits [percent coefficient of variation was less than 15%]. Sample preparation briefly involved 0.4 μ membrane filter through which the sample was filtered, diluted with mobile phase, and 10 μL was spiked into
The voltammetric measurement has been performed by transferring the oxidized TMB solution to the 96X carbon electrode. Thus, linear sweep voltammetry were measured by sweeping the potential from +6.00 to -2.00 mV. The calibration curve couldn 't be plotted with the obtained voltammograms, therefore, the cocaine-benzoylecgonine antibody concentration has been raised to 0.05 µg/ml (10 fold more) and the tracer dilution ratio to 1:10000. That leads to increase the absorbance (OD) detected by ELISA to 4. The obtained voltammograms were investigated and the calibration curve was plotted.
This product is a good choice to buy and it is a healthy choice. Something similar to smart water is Eternal Naturally Alkaline Water. Eternal Water is Naturally Alkaline, which means the water pH is in a basic state, ranging from 7.8 - 8.2. Naturally Alkaline is rare and unique which has underground spring sources that are Naturally Alkaline. The mineral properties of the water are not artificially manipulated.
The titrations were all a bit different and that was because they all contained different liquids. The first titration, acid into water, showed a noticeable drop in ph after approximately 5.5 mL of acid was added and was consisted in showing low numbers. This determined that the first hypothesis, stating that the pH would go up when acid was added into water, was false. Dissimilarly to the second titration, base into water, the pH showed a noticeable increase after around 10 mL of base was added and once again, remained consistently high in pH. The third titration, acid into liver homogenate, the pH decreased a bit at 8 mL of acid, but remained fairly consistent at 12 mL.
The beginning reaction that occurred at the pH level of 1 shows that the mean reaction rate was incredibly low, at 2 mL/minute. This then increased by 57 units once it reached its peak productivity of 59 mL/minute observed at pH 8. pH levels 6, 7, and 8 only varied between 1 and 2 mL/minute, which demonstrated similar rates of reaction. At pH 10, the reaction rate decreased considerably as it declined by 58 mL/minute, and maintained that productivity at pH 12. The scatter graph included in the results section further solidify and visually represent these observations. The reaction rate of the catalase exposed to pH 1 is barely conceivable on the diagram as its average rate of reaction was 2 mL/minute.