Repeat step four for each sample with a new sterile swab each time. 6. Label the petri dishes according to their samples, and seal each with tape. 7. Then, to take data, each day place the 0.5cm^2 piece of grid paper underneath the petri dish and count the approximate number of bacteria in one
Using the lit tea light, like a bursen burner, I flamed the mouth of the S. epidermidis culture. The sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used.
Materials and Methods To start with, the unknown bacteria # 710 broth had to be successfully isolated on an EMB and MAC agar plate. Using aseptic technique by sterilizing the wire loop with Bunsen Burner between inoculations and flaming the opening of the test tubes before inserting in the loop with the bacteria. The streaking technique used was to isolate the colonies on the agar plates. In addition, the streak plates had to be incubated in a upturned position for 24 hours in a hot temperature incubator at 37 degree Celsius. Bacteria need a favorable condition to grow in.
Make a garlic and water mixture. Apply some amount of mixture to the wart and cover it with bandage. Make sure to reapply for several hours and continue until the wart is gone. Make a paste out of baking powder and castor oil. Apply at night on warts and cover a bandage.
Extra care was taken to not touch the plate with bare skin. Five spots were labeled on the line and each amino acid standard was spotted on the plate using a capillary tube. The standards included leucine, alanine, phenylalanine, and lysine. The final spot was an unknown mixture of three amino acids. After allowing the spots to dry, the plate was placed in the developing jar and allowed to develop.
(1;p 326) Method I collected 1/8 c of saliva, then added the following: 2 drops of dish detergent (stirred 30 sec), 2 drops of contact lens solution (stirred 30 sec), and a small pinch of table salt (stirred 30 sec). Then, I tilted the cup approximately at a 45 degree angle and ran ¼ c of the ice cold rubbing alcohol down the side of the cup, which formed a layer on the top of the saliva-soap solution. (no mixing). Once the solution separated I then took two sticks and wrapped the DNA around them by gently spinning the sticks in the school with the DNA streaks. Final step was mailing the DNA sample to Tory Blackwell for PCR analysis.
Name: Nisha Ghayalod Drawer/Group #: G2 PS ID #: 1257853 Three digit mutant code: 651 BIOL 3311 Fall 2016 Lab Section: 5-digit number 19524 Date: 11 September 2016 TA Instructor Name: Rintu Thomas Description of Unknown Mutant Allele Phenotype Drosophila melanogaster (fruit flies) are organisms that contain multiple types of mutations. A few examples of these mutations involve eye color, wing formation, body size and body color. When comparing the wild type version of D. melanogaster to mutant D. melanogaster with unknown code 651, it was shown that the mutant contained a mutation in the bristles located on the fly’s thorax and head. These mutated flies have short and curly bristles (Figure 1, A arrows) compared to their wild type counterparts which have long and straight bristles (Figure 1, B arrows) in the same locations. This mutation has led us to
Research Protocol – Monitoring of the Daphnia magna heart rate The experiments focused on the four treatments of nicotine, caffeine, ethanol, and double distilled water (placebo). 180 μL of each bioactive solution (nicotine was covered with foil, due to light sensitivity) and 120 μL of double distilled water were placed into labeled eppendorf tubes to dismiss cross-contamination, and were placed on ice to match the environment of the Daphnia to reduce any added stress on the Daphnia. One Daphnia was placed on the testing petri dish, and then all the excess pond water was removed with a transfer pipette. Immediately 10 μL of double distilled water was added with a micropipette; this way our concentration of the treatment was the intended concentration.
We let the potato cores sit in the solution overnight. The next day we then emptied the beaker of the solution by carefully draining the solution, while not letting the potato cores fall out. We then took the potato cores out of the empty beaker and dabbed them lightly with paper towel to get any excess solution off. We did this quickly and following it we then took the mass of all four potato cores again and recorded
The testing for affectability of a life form to antimicrobial agent is normally done utilizing agar dissemination or disk diffusion test. The parameters of this test were indicated (or institutionalized) by the researchers W. M. M. Kirby and A. W. Bauer and is likewise alluded to as the Kirby-Bauer antibiotic testing. In this technique, anti-toxins or antibiotic are impregnated on a specific extraordinary kind of paper circles and are put on the surface of agar containing the bacterium and parasitic (fungi) of our interest. This outcomes in the dispersion of antimicrobial agent into the surrounding medium. The diameter of the zone of inhibition will decide the adequacy or sensitivity of the antibiotic; the bigger the diameter, the more