Through enzyme digestion experiments, I am able to understand the role of restriction enzymes in bioengineering. And I can clearly see the length of the fragment when the restriction enzyme cleaves DNA. This experiment also made me gain knowledge about DNA analysis. Through the experiment of Restriction Endonuclease Digestion of DNA, I learnt that when using RedSafe stain and the UV light source, I should be careful because their mutagenic effects. Moreover, this experiment makes me understand the role of gel electrophoresis in DNA preparation and analysis.
This essay will look more on the advantages and disadvantages of genetic engineering. Genetic engineering has some advantages. Firstly it allows for faster growth rate .Genetic engineering allows of plants or animals to be modified so their maturity can occur at a quicker pace outside of the normal growth conditions that are favourable without genetic changes as well .Secondly, it may also provide a cure for disease to improve health (apecsecadmin, 2014). Genetic engineering can be used to eradicate a number of incurable and deadly disease which could be done by identifying the genes that cause these disease, and manipulate them to prevent people from contracting these potentially deadly diseases. Genetic engineering could theoretically eliminate the passing of “disease” genes.
Amir Ahemedin Ms.Buckley Genetics 11/06/15 Transformation of E.coli Lab Purpose The purpose of this lab is to genetically engineer the E.coli strain by introducing two genes, the green fluorescent protein gene (GFP) and the ampicillin resistant gene (AMP). Then observe whether or not the E.Coli strain would take up these genes and become fluorescent. Background Information In this lab, bacterial transformation was one of the three processes that occurred when genetic material is introduced to a bacterial cell. Bacterial transformation is important because it allows for the cloning and movement of DNA between strains. This transformation usually occurs within plasmids, which are closed circular molecules made up of double stranded DNA.
Genetically modified animals involve the changing of the genetic information by adding or removing DNA sequences in an uncommon way that is not natural. Genetic modification aims to modify certain characteristics of animals and/or introduce new traits. Those traits include resistance of diseases and growth enhancement (Ormandy, Dale, & Griffin, 2011). Keyword(s): genetic modification What is Genetic Modification? Genetic modification is the manipulation of genes through gene isolation, modification of genes so that they function better, preparation of genes that are inserted into new species, and transgene development.
This mutation occurs at a point in the DNA sequence that is recognized by the restriction enzyme MstII in a person without the disease. RFLP of a person suffering from sickle cell disease have a single long band instead of two shorter bands because MstII cleavage will not likely to occur. Mutations in DNA between species are investigated by RFLP analysis. Figure 6:" Sickle cell anemia detected by RFLP analysis" RFLPs can also be employed in different configurations to achieve various objectives: • RFLPs can be used in criminal cases or paternity cases to determine the origin of a DNA sample (forensic
Several issues have arisen in terms of whether it is beneficial to humans, or if it does more harm than good. While there are some areas of concern when it comes to genetic modification, this technology is beneficial to humans because it allows several industries to make more income, and it helps prevent several illnesses that can cause serious harm to humans. It is important to know the context of genetic engineering before discussing the benefits of it to the public. Genetic engineering, also known as genetic modification, is the process of altering the DNA in an organism. There are several processes that constitute genetic engineering, and it can range from changing one base pair of DNA to combining the DNA from another organism.
G This essay is going to present the issue of genetic engineering with its pros and cons. Genetic engineering is the modification of an organism’s genetic composition by artificial means which often involves the transfer of specific traits from one organism into a plant or animal of an entirely different species (Grace Communications Foundation. ND). The results of the process are called GMO (Genetically modified organism) or a transgenic. Genetic engineering enables the creation of animals, plants by changing the genes in a way that does not occur naturally in order to obtain desired traits.
A prophecy is a prediction of what will happen in the future. With advancements in real world we could accomplish this goal. Frankenstein is a prophecy because we are heading in that direction with the help of technology, however we would handle our creations much better than Victor. Progress has made us get to this point in life, while in Frankenstein they have more advancements, but we have learned to do plastic surgery and have created antibiotics. Joe Rosen states that “we use antibiotics to combat the flu” antibiotics have help us now combat not the only flu but other diseases, and illnesses so humans would not have to suffer from these illnesses (Rosen 17).
If gene manipulation is not intrinsically wrong, should we still do it? Mr. Sandler views gene manipulation, or gene drive, as a new conservation tool that can create additional methods of approaching conservation issues. He argues that the gene drive method often meets key conservation criteria better than traditional methods. Genetic manipulation is already used to influence the longevity of certain species, namely to reduce the impact of invasive organisms. However, the genes of the actual species at risk can also be manipulated.
How do this modifications affect the genes? It all start when the genes carry the blueprints to make proteins in the cell. The DNA sequence of a gene is transcribed into RNA, which is then translated into the sequence of a protein. Because they change how genes can interact with the cell 's transcribing machinery, epigenetic modifications, generally turn genes on or off, allowing or preventing the gene from being used to make a protein. On the other hand, mutations and bigger changes in the DNA sequence like deletions change not only the sequence of the DNA and RNA, but may affect the sequence of the protein as well.
From that point on, new nucleotides are added to each of the original strands (A to T, C to G) until the result is two identical sequence copies of DNA. 3. How is DNA information used to synthesize polypeptides? A gene or protein is used to make polypeptides. In order to create this gene, transcription and translation must take place to create a protein from DNA.
Genetically modifying food is a relatively new method of enhancing plants as genes are transferred between organisms. Once the desired gene is identified in the organism’s genome, it can be cut out, transferred to the target plant, and pasted into its genome. Many authors have written about scientists who attempt to transform nature and its entities. For example, in Nathaniel Hawthorne’s “The Birthmark” and “Rappaccini’s Daughter,” organisms are modified throughout the respective stories. Essentially, the modification of each individual in the two works results in detrimental effects such as death and poisoning caused by toxic substances.
The topics that he mentions involve things such as genetic manipulation as well as cloning. He begins by mentioning how this has been simply used for the growth of plants and crops, but he questions where this is potentially leading and how the future of the environment could potentially be destroyed in many ways. He makes a strong argument about this by saying, "But by doing these things, we are changing the genetic makeup, and do we really know what the long-term impact will be on the species of plants, on the soil, on the environment? There are obvious commercial benefits, but how do we judge what is really useful?" (133).
Genomic Recombination and Deletions in Acinetobactor baylyi ADP1 Shivani Patel Fall 2015 BIO 493 Introduction: Gene duplication and amplification is a process by which genetic diversity can be created and selected for. Through the understanding of gene duplication and amplification, scientists can garner insight on medical conditions associated with this phenomenon (Seaton et al. 2012). Not only can gene duplication and amplification increase genetic diversity, it can also increase the fitness of bacteria by allowing an increased production of essential nutrients or a gene to gain a new function (Dhar et al. 2014).